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Plasmid#161919PurposeExpresses human TK1 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.DepositorInsertThymidine kinase, cytosolic (TK1 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-2
Plasmid#52378Purposeexpresses human Syndecan-1 GAGAL replaced with GADEV of SDC2 in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationGAGAL of syndecan-1 replaced with of GADED of SDC…PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMIG-AVITEV- hNFATc1/aA
Plasmid#74057Purposeretroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc1, isoform alpha-A (NFATC1 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianMutationmutation G1157A [R235Q] and the silent mutation C…PromoterpMSCV-LTRsAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
BAP1-010
Plasmid#68366Purposestable expression of BAP1 delta 221-238 in mammalian cellsDepositorInsertBAP1 (BAP1 Human)
UseTagsExpressionMammalianMutationdelta 221-238, splice variant found in human meso…PromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 S387A
Plasmid#59905PurposeExpresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationS387A, phospho-null alaninePromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-4
Plasmid#52375Purposeexpresses human Syndecan-1 GAGAL replaced with GDLDD of SDC4 in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationGAGAL of syndecan-1 replaced with GDLDD of SDC4; …PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 5A
Plasmid#60651PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationmutated five serines to alaninesPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pRSF-DUET1-DHDDS/Sumo-NgBR
Plasmid#203162PurposeExpression of human cis-prenyltransferase complex for purificationDepositorUseTagsHis6-SUMOExpressionBacterialMutationtruncationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ICOSLG-COMP5AP-AviTag-9xHis
Plasmid#157254PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertICOSLG (ICOSLG Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+HIF1a-AS1
Plasmid#194172PurposeExpresses lncRNA HIF1a-AS1 (spliced)DepositorInsertHIF1a-AS1 (HIF1A-AS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 4A
Plasmid#59907PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTags6xHis (N-termi tag)ExpressionMammalianMutationmutated four serines to alaninesPromoterCMVAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223-LOC390877
Plasmid#23373DepositorInsertLOC390877 (LOC390877 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-NGN2-BRN3A
Plasmid#165597PurposeDonor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.DepositorInsertsUseCRISPR and TALENTagsExpressionMammalianMutationPromoterCAG, EF-1alpha, Endogenous CLYBL (splice acceptor…Available sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CFLAR_WT_V5
Plasmid#82936PurposeGateway Donor vector containing CFLAR, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertCFLAR (CFLAR Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ICOSLG-Fc(DAPA)-AviTag-6xHis
Plasmid#156690PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertICOSLG (ICOSLG Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDCAF3-IS5
Plasmid#65220PurposeExpresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.DepositorInsertsndmA
ndmB
ndmC
ndmD
gst9
chlR
UseSynthetic BiologyTagsExpressionBacterialMutationChanged start codon from gtg to atg and Changed s…PromoterBBa_J23100Available sinceJan. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223_CASP8_WT_V5
Plasmid#82999PurposeGateway Donor vector containing CASP8, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertCASP8 (CASP8 Human)
UseGateway entry vectorTagsExpressionMutationAA 184_191delERSSSLEGinsGEELCGVMTISD; D194R; 196_…PromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only