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Plasmid#214839PurposeThis plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-30nmSAH-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys
Plasmid#90473PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertTagGFP2-TEV-GGG-ELP(120nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNR188
Plasmid#79543PurposeOutput plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Myr.PKCalpha.FLAG
Plasmid#10807DepositorInsertPKC alpha (PRKCA Bovine)
UseTagsFLAG and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
Non tagged Avidin
Plasmid#214835PurposeThis plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertNon tagged Avidin
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDmDERusk
Plasmid#213702PurposeVector for red-light-induced bacterial gene expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSVBneoT
Plasmid#22883DepositorInsertEarly region SV40
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP20
Plasmid#22573DepositorInsertUSP20 (USP20 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUB-GFP
Plasmid#11155PurposeMammalian expression vector for expression of GFP (Ubiquitin C promoter)DepositorUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTaU6-esgRNA
Plasmid#115630Purposeexpression esgRNA in wheat protoplastsDepositorInsertTaU6p-esgRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUG-124
Plasmid#104635PurposeLevel 2 constructDepositorInsertHIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_EL4
UseTagsHIS-Ubq, HA-E2, GST-E3ExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP39
Plasmid#22581DepositorInsertUSP39 (USP39 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP_LEU2D
Plasmid#58352PurposeUsed for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiaeDepositorTypeEmpty backboneUseTagsGFP-HisExpressionYeastMutationPromoterGAL1Available sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-nanoHER2-SpyTag003
Plasmid#216312PurposeExpresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.DepositorInsertnanoHER2-SpyTag003
UseTagsHis6 tag and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM43 p62 FL (1-440)
Plasmid#210425PurposeBacterial expression of MBP-p62 FL (1-440) with 3C cleavage siteDepositorInsertp62 FL (NUP62 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBB535
Plasmid#27392DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMF230
Plasmid#62546PurposeBroad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.DepositorInsertGFP mut2
UseBroad host range plasmidTagsExpressionMutationPromotertrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEVA1213S
Plasmid#122095Purposeconstitutive expression of I-SceI for genome engineering in PseudomonasDepositorInsertI-SceI restriction endonuclease
UseTagsExpressionMutationPromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG413 GAL ORF1 Shuffle1 NLS
Plasmid#145794PurposemPing Yeast Transposition AssayDepositorInsertORF1 Shuffle1 NLS
UseTagsExpressionYeastMutationEnhanced Nucleur Localization SignalPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -