-
Plasmid#140145PurposeTetracycline-inducible TEADi (GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal)DepositorInsertEGFP TEADi
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2
Plasmid#149517PurposeN-termial APEX2 tag. Gateway destination vector for mammanlian expressionDepositorTypeEmpty backboneUseTagsFLAG-APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDP085
Plasmid#198425PurposepUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120DepositorInsertE. coli BirA
UseTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Bin1b
Plasmid#109509PurposeMuscle specific zebrafish expression of mKate2 fused to Bin1bDepositorInsertBin1b (bin1b Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-VP64
Plasmid#50916PurposeTet-regulatable dCas9-VP64 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and VP64ExpressionMammalianMutationD10A, H840A nuclease-deficientPromoterTREAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-luc2
Plasmid#194384PurposeReporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoterDepositorInsert6xCRE-CMVmin-luc2
UseTagsExpressionMammalianMutationPromoterCMV minimalAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL6
Plasmid#67061PurposeStandard for cell-free expression benchmarking.DepositorInsertIL6 (IL6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-KIF20A zeo
Plasmid#192322PurposeLentiviral expression vector for an inducible 3xFLAG-KIF20ADepositorInsertKIF20A (KIF20A Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
HLCS_pCSdest
Plasmid#53803DepositorInsertHLCS (HLCS Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ143-ZmUbi-tRNA
Plasmid#158400PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-NDP52
Plasmid#175749PurposeLentiviral vector expressing N-terminally tagged eGFP-NDP52/CALCOCO2DepositorInsertNDP52/CALCOCO2 (CALCOCO2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMALB
Plasmid#161786PurposeGateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.DepositorTypeEmpty backboneUseLentiviralTagsTagBFPExpressionMammalianMutationPromoterBidirectional minhCMV and SFFVAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_EPHB2
Plasmid#187797PurposeMAC-tagged gene expression of human EPHB2DepositorInsertEPHB2 (EPHB2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti X1 Puro DEST (694-6)
Plasmid#17297Purpose3rd gen lentiviral promoter-less Gateway destination vector, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAi; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_ELAVL1
Plasmid#106104PurposeInducible gene expression of FlagHA ELAVL1DepositorInsertELAVL1 (ELAVL1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP11-pDEST-C
Plasmid#118367PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14_sur5p-dCas9-VP64
Plasmid#177788PurposeOverexpression of dCas9-VP64 in C. elegansDepositorInsertdCas9-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-N
Plasmid#118368PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-AdEvolve-DEST
Plasmid#131692PurposeA version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.DepositorTypeEmpty backboneUseAdenoviralTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycDDX17
Plasmid#19876DepositorInsertDEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only