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Plasmid#63729PurposeTo express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).UseTagsT2AExpressionMammalianMutationcodon optimizedPromoterSFFVAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only
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pN1 Lck-GCaMP5G
Plasmid#34924PurposeExpresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membraneDepositorInsertLck-GCaMP5G
UseTagsExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…PromoterCMVAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
TRE-mClover3-TDP-43ΔNLS
Plasmid#214917Purposeinducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear punctaDepositorInsertTDP-43ΔNLS (TARDBP Human)
UseLentiviralTagsmClover3ExpressionMutationmutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…PromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRE-TDP-43ΔNLS-mClover3
Plasmid#214916Purposeinducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43DepositorInsertTDP-43ΔNLS (TARDBP Human)
UseLentiviralTagsmClover3ExpressionMutationmutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…PromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_3xFLAG_V5_ mmIfih1_V5
Plasmid#160110PurposeExpresses murine Ifih1 in mammalian cellsDepositorInsertIfih1 (Ifih1 Mouse)
UseLentiviralTags3xFLAG, V5 and V5ExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)
Plasmid#55614PurposeDox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cellsDepositorInsert3xFLAG tagged HERC2 (C4762S) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationC4762 mutated to serine to render HERC2 catalytic…PromoterAvailable sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-S15/25A
Plasmid#52364Purposeexpresses human Syndecan-1 serine 15 and 25 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationS15A, S25A and R95Q (according to numbering in Fi…PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTT5-hSDC1-N20
Plasmid#52355Purposeexpresses human syndecan 1 'del 1- 20' truncation mutant in HEK293-EBNA1 (293E) suspension culture.DepositorInsertSDC1 (SDC1 Human)
UseTagsExpressionMammalianMutationDeletion of SDC1 aa2-21 (according to numbering i…PromoterCMVAvailable sinceDec. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUDP002
Plasmid#103872PurposeBroad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)DepositorInsertshph
Spcas9 D147Y P411T
UseCRISPRTagsNLSExpressionYeastMutationD147Y P411TPromoterArxula adeninivorans TEF1 and Ashbya gossypii (Er…Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170995PurposeHiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)DepositorInsertAKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
lucMAPT-30D
Plasmid#214672PurposeMAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice siteDepositorInsertMAPT (MAPT Human)
UseTagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9, Exon 10 with a mutation to intro…PromoterCMVAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
lucMAPT-30U
Plasmid#214673PurposeMAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice siteDepositorInsertMAPT (MAPT Human)
UseTagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9, Exon 10 with a mutation to intro…PromoterCMVAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRM15
Plasmid#163694Purposehsp70 promoter inducible expression of DHB:mScarlet-P2A-H2B:miRFP670DepositorUseZebrafish expressionTagsmScarlet and miRFP670ExpressionMutationPromoterHSP70Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Blast-DNFGFR1-HA
Plasmid#130887PurposeExpresses dominant negative mutant mouse FGFR1DepositorInsertFgfr1 (Fgfr1 Mouse)
UseTagsHA-tagExpressionMammalianMutationDominant Negative receptor lacking cytosolic cata…PromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRM14
Plasmid#163693Purposehsp70 promoter inducible expression of DHB:mNeonGreen-P2A-H2B:mScarletDepositorUseUnspecified; Zebrafish expressionTagsmNeonGreen and mScarletExpressionMutationPromoterHSP70Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3Basic-ME.2/ApoEpromoter
Plasmid#51436PurposeThis plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancerDepositorUseLuciferaseTagsExpressionMammalianMutationThe ME.2 enhancer is fused upstream of the ApoE g…PromoterAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3Basic_ME.1/ApoEpromoter
Plasmid#51435PurposeThis plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancerDepositorUseLuciferaseTagsExpressionMammalianMutationME.1 enhancer is fused upstream of ApoE promoter …PromoterAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) FLAG-Ago2 S387E
Plasmid#60653PurposeExpresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTagsCMV-FLAG-Ago2 (N-term tag)ExpressionMammalianMutationS387E mutantPromoterCMVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a_His-TEV-EiCsm6
Plasmid#172487PurposeBacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.DepositorInsertEnterococcus italicus Csm6 (codon optimized)
UseTagsHis6ExpressionBacterialMutationPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 (+) FLAG-Ago2 S387A
Plasmid#60652PurposeExpresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
UseTagsCMV-FLAG-Ago2 (N-term tag)ExpressionMutationS387A mutantPromoterCMVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only