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Plasmid#149517PurposeN-termial APEX2 tag. Gateway destination vector for mammanlian expressionDepositorTypeEmpty backboneUseTagsFLAG-APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only
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pENTR221 UBR5
Plasmid#81062PurposeGateway entry vector encoding full length UBR5DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) (UBR5 Human)
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
APEX2-GBP
Plasmid#67651PurposeMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).DepositorInsertAPEX2-GBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL6
Plasmid#67061PurposeStandard for cell-free expression benchmarking.DepositorInsertIL6 (IL6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ143-ZmUbi-tRNA
Plasmid#158400PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Hygro DEST (w117-1)
Plasmid#17454Purpose3rd gen lentiviral Gateway destination vector, expression, CMV promoter, HygroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Hygro DEST (w530-1)
Plasmid#19066Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, HygroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSMALB
Plasmid#161786PurposeGateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.DepositorTypeEmpty backboneUseLentiviralTagsTagBFPExpressionMammalianMutationPromoterBidirectional minhCMV and SFFVAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_ELAVL1
Plasmid#106104PurposeInducible gene expression of FlagHA ELAVL1DepositorInsertELAVL1 (ELAVL1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-NDP52
Plasmid#175749PurposeLentiviral vector expressing N-terminally tagged eGFP-NDP52/CALCOCO2DepositorInsertNDP52/CALCOCO2 (CALCOCO2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-luc2
Plasmid#194384PurposeReporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoterDepositorInsert6xCRE-CMVmin-luc2
UseTagsExpressionMammalianMutationPromoterCMV minimalAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_EPHB2
Plasmid#187797PurposeMAC-tagged gene expression of human EPHB2DepositorInsertEPHB2 (EPHB2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-VP64
Plasmid#50916PurposeTet-regulatable dCas9-VP64 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and VP64ExpressionMammalianMutationD10A, H840A nuclease-deficientPromoterTREAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP1-10-pDEST-N
Plasmid#118368PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP11-pDEST-C
Plasmid#118367PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-AdEvolve-DEST
Plasmid#131692PurposeA version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.DepositorTypeEmpty backboneUseAdenoviralTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14_sur5p-dCas9-VP64
Plasmid#177788PurposeOverexpression of dCas9-VP64 in C. elegansDepositorInsertdCas9-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Hygro DEST ERKKTRmCerulean3
Plasmid#90229PurposeLentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLenti X1 Puro DEST (694-6)
Plasmid#17297Purpose3rd gen lentiviral promoter-less Gateway destination vector, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAi; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only