-
Plasmid#50483Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsYFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the YFP open…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_Y357F-PolyA
Plasmid#112287PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoformDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationTyrosine 357 to Phenyalanine corresponding to tyr…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-NLS-VP16
Plasmid#103822PurposeSoluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-EYFP-VP16 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationVP16: G126T (silent, G42G)PromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
p35S::ARA6/RabF1-YFPc
Plasmid#102408Purposesplit YFP. Plant expression of p35S::ARA6/RabF1-YFPcDepositorInsertARA6 (ARA6 Mustard Weed)
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-R514S-YFP
Plasmid#29617DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationR514SPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL VV458/460PP
Plasmid#61368PurposeExpresses Human full length RIPK3 with A458P/V460P mutation in the mammalian cellsDepositorInsertFull length human RIP3 with VV458/460P mutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged V458 to P and V460to PPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b/EYFP-GgVcl 1-258
Plasmid#46263DepositorInsertVcl 1-258 (VCL Chicken)
UseTagsEYFP and HisExpressionBacterialMutationaa 1-258 (VD1)PromoterT7Available sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4r-EYFP-R3r
Plasmid#32412DepositorInsertEYFP
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP mouse Rab35 WT
Plasmid#53517DepositorInsertRab35 (Rab35 Mouse)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP mouse Rab35 CA
Plasmid#46015DepositorInsertRab35 (Rab35 Mouse)
UseDrosophilaTagsYFPExpressionInsectMutationQ67L (constitutively active)PromoterGAL4Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-R524S-YFP
Plasmid#29626DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationR524SPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-R522G-YFP
Plasmid#29625DepositorInsertFUS (FUS Human)
UseTagsYFPExpressionYeastMutationR522GPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
Aquamarine-LC3B G120A-TdLanYFP
Plasmid#228561PurposeEncodes an uncleavable version of the LC3B biosensor. Mutated LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoterDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseTagsAquamarine and TdLanYFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-Bcx
Plasmid#191195PurposeFusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & Bcx fusion
UseTags6xHis and Bacillus circulans xylanaseExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-mScarlet-i
Plasmid#191196PurposeFusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & mScarlet-I fusion
UseTags6xHis and mScarlet-IExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-SAV
Plasmid#191197PurposeFusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & Streptavidin fusion
UseTags6xHis and Streptavidin core domain, amino acids 1…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TOgw-C-mEYFP
Plasmid#223951PurposeGateway cloning of genes of interest with a C-terminal mEYFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmEYFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUSE-URA3-Sec7-mEYFP
Plasmid#218968PurposeGene replacement plasmid to label S. cerevisiae Sec7 with mEYFPDepositorInsertSEC7-mEYFP (SEC7 Budding Yeast)
UseGene replacementTagsmEYFPExpressionMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUSE-URA3-Sec7-msYFP2
Plasmid#218969PurposeGene replacement plasmid to label S. cerevisiae Sec7 with msYFP2DepositorInsertSEC7-msYFP2 (SEC7 Budding Yeast)
UseTagsmsYFP2ExpressionYeastMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-HM4Di-YFP
Plasmid#209193PurposeCre-dependent expression of Hm4Di fused to YFP; ChemogeneticsDepositorInsertHm4Di
UseAAVTagsYFPExpressionMammalianMutationPromoterCAGAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only