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pcDNA3.1-R1ACR_367-EYFP

Plasmid

#103773

Purpose

Anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R1ACR_367

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GcACR_439-EYFP

Plasmid

#103142

Purpose

Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin GcACR_439

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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T660K EPHA3 pcDNA3.1

Plasmid

#102749

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

T660K (ACA > AAA)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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S449F EPHA3 pcDNA3.1

Plasmid

#102748

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

S449F (TCC > TTC)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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A435S EPHA3 pcDNA3.1

Plasmid

#102747

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

A435S (GCT > TCT)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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N379K EPHA3 pcDNA3.1

Plasmid

#102745

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

N379K (AAT > AAG)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
T166N EPHA3 pcDNA3.1

Plasmid

#102742

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

T166N (ACT > AAT)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
D806N EPHA3 pcDNA3.1

Plasmid

#102754

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

D806N (GAT > aAT)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
K761N EPHA3 pcDNA3.1

Plasmid

#102752

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

K761N (AAG > AAt)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
R728L EPHA3 pcDNA3.1

Plasmid

#102751

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

R728 (CGA > CtA)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
T37K EPHA3 pcDNA3.1

Plasmid

#102740

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

T37K (ACA > AAA)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
N85S EPHA3 pcDNA3.1

Plasmid

#102741

Purpose

Expression of EPHA3 in mammalian cells

Depositor

Jin Chen

Article

Zhuang et al J Natl Cancer Inst. 2012 Aug 8;104(15):1

Insert

EPHA3 (EPHA3 Human)

Use

Tags

myc-6xhis

Expression

Mammalian

Mutation

N85S (AAC > AGC)

Promoter

CMV

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _1-480

Plasmid

#86817

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mCherry Gamma 3

Plasmid

#65234

Purpose

To study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cell

Depositor

Narasimhan Gautam

Article

Giri et al Biophys J. 2014 Jul 1;107(1):242-54. doi

Inserts

mcherry
Gng3 (Gng3 Mouse)

Use

Tags

6-His tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-a4-delta tail

Plasmid

#80018

Purpose

Mammalian expression of human integrin alpha4 with no tail

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Use

Tags

Expression

Mammalian

Mutation

no cytoplasmic tail (amino acids 1 to 1007)

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Alpha-0 Dronpa

Plasmid

#44193

Depositor

Narasimhan Gautam

Article

Chisari et al J Biol Chem. 2007 Aug 17;282(33):24092-8

Insert

G protein alpha 0 (Gnao1 Rat)

Use

Tags

Dronpa

Expression

Mammalian

Mutation

Introduced KpnI and SpeI sites between 92nd and 9…

Promoter

CMV

Available since

April 14, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7ADelta2bp-YFP

Plasmid

#62637

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A an…

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hmga2 shMut wt

Plasmid

#52036

Purpose

encodes shMut HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

shRNA binding site mutated

Promoter

CMV

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Hmga2 shMut m7

Plasmid

#52037

Purpose

encodes shMut HMGA2 m7

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

shRNA binding site mutated, all seven let-7 sites…

Promoter

CMV

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-hPMCA4b-D672E

Plasmid

#47596

Depositor

Emanuel Strehler

Insert

PMCA4b-D672E (ATP2B4 Human)

Use

Tags

Expression

Mammalian

Mutation

D672E (reducing the ATPase activity to less than…

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only