We narrowed to 5,554 results for: pCas

1. pCAS9-BFP empty

   Plasmid

   #80976

   Purpose

   Cas9, TagBFP, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-GST-ATG14

   Plasmid

   #203566

   Purpose

   Mammalian expression of GST tagged ATG14

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG14 (ATG14 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBT224_(pCA-tTA2)

   Plasmid

   #36430

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Insert

   tetracycline transactivator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. G1397 DddAtox-N–dSpCas9

   Plasmid

   #157835

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9 TadCBEa-V106W

   Plasmid

   #193838

   Purpose

   Express TadCBEa-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDa V106W-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Bmpr2Δ751-813aa

   Plasmid

   #163603

   Purpose

   Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmpr2 (Bmpr2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The LIMK-binding region (751-813 aa) is deleted

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Gal4DBD-GBP2

   Plasmid

   #49439

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP2-Gal4 DNA binding domain fusion protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-3xFLAG-Rbfox2

   Plasmid

   #213726

   Purpose

   Mammalian cell expression vector for overexpression of Rbfox2 in motor neurons.

   Depositor

   Chaolin Zhang

   Article

   Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

   Insert

   Rbfox2 (Rbfox2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAX APP delta CT

   Plasmid

   #30143

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion of C-terminal intracellular domain (afte…

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-nSpCas9-TadA8e

    Plasmid

    #209044

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TadA8e-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)_No_FLAG

    Plasmid

    #79877

    Purpose

    FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-CD4-myc

    Plasmid

    #58537

    Purpose

    Expresses human CD4 with a C-terminal myc tag in mammalian cells

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    CD4 (CD4 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCasper_Pdf-LexA::VP16

    Plasmid

    #33259

    Depositor

    Michael Rosbash

    Article

    Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;10

    Insert

    LexA::VP16-SV40 3'UTR (lexA E. coli)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Pdf

    Available since

    Dec. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCAG_Xph15-eGFP-CCR5TC

    Plasmid

    #135528

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG vGlut1 GCaMP5G

    Plasmid

    #105008

    Purpose

    Presynaptic calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    SLC17A7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Ace2_D92N_E199V-mNeon

    Plasmid

    #129256

    Purpose

    Express Ace2_D92N_E199V-mNeon in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace2_D92N_E199V-mNeon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAX APP C60

    Plasmid

    #30152

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein C60
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fragment

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-nDsRedFL-intron

    Plasmid

    #177804

    Purpose

    An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    intron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. hGli3 NTOTAL in AINGpCAGGS

    Plasmid

    #121973

    Purpose

    medium Gli3 transcriptional repressor

    Depositor

    James Briscoe

    Article

    Ulloa et al Curr Biol. 2007 Mar 20;17(6):545-50. doi

    Insert

    hGli3 NTOTAL (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc and Linker A IRES NLS GFP pCAGGS

    Expression

    Mammalian

    Mutation

    C terminus deletion from zinc finger domain

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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