We narrowed to 5,857 results for: pCas

1. pCAG_Xph15-SNAPf-CCR5TC

   Plasmid

   #135536

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Tags

   SNAPf

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCambia2300 - ProRUBY:RUBY-Citrine

   Plasmid

   #128796

   Purpose

   Fluorescent reporter for subcellular localisation and expression analysis of RUBY

   Depositor

   George Haughn

   Article

   Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

   Insert

   RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)
   

   Tags

   Citrine

   Expression

   Plant

   Promoter

   ProRUBY

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

   Plasmid

   #86612

   Purpose

   Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via nhej using ouabain

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCARGO-RMCE-Xist

   Plasmid

   #233268

   Purpose

   CARGO plasmid containing ~22kb dox-inducible Xist transgene

   Depositor

   Mauro Calabrese

   Article

   Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

   Insert

   22kb Xist gene, including introns
   

   Expression

   Mammalian

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-NRCH-SsAPOBEC3B

   Plasmid

   #198550

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-SsAPOBEC3B
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas-MmdCas12m-CBE1

   Plasmid

   #192284

   Purpose

   MmdCas12m-CBE1 expressed under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmdCas12m-CDA-UGI
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D485A

   Promoter

   PJ23108

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ER50-SpCas9-ER50

   Plasmid

   #85448

   Purpose

   Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   ER50-SpCas9-ER50
   

   Use

   AAV

   Tags

   destabilized domain of estrogen receptor ligand b…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-mCherry2-mito

   Plasmid

   #164164

   Purpose

   Expresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoter

   Depositor

   Isei Tanida

   Article

   Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

   Insert

   mCherry2-mtActA
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCasper4-g-osk-TRICK

   Plasmid

   #71244

   Purpose

   oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   oskar (osk Fly)
   

   Use

   P element-mediated germline transformation

   Tags

   12xPP7 and 6xMS2

   Expression

   Insect

   Promoter

   osk

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6-hGRIN2B-CAG-ps-SpCas9

    Plasmid

    #102851

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-CoChR-mRuby2-ST

    Plasmid

    #109131

    Purpose

    Cation channelrhodopsin CoChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    CoChR-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-b3STOPb3-ZsGreen

    Plasmid

    #51266

    Purpose

    B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    b3STOPb3-ZsGreen
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Myc-Oct3/4-IP

    Plasmid

    #13460

    Depositor

    Shinya Yamanaka

    Article

    Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Tags

    Myc (6x)

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-OSF-VPS15

    Plasmid

    #99326

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    VPS15 (PIK3R4 Human)
    

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-memRFP-3xControlgRNA

    Plasmid

    #224567

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG/ATP10D(E215Q)-HA

    Plasmid

    #209248

    Purpose

    Mammalian expression of ATP10D mutant E215Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10D (ATP10D Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E215Q

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNS20-SpCas9-SNAP

    Plasmid

    #113717

    Purpose

    Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG/ATP9A(E195Q)-HA

    Plasmid

    #209244

    Purpose

    Mammalian expression of ATP9A mutant E195Q

    Depositor

    Hye-Won Shin

    Article

    Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

    Insert

    ATP9A (ATP9A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E195Q

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAS9-BFP empty

    Plasmid

    #80976

    Purpose

    Cas9, TagBFP, and sgRNA expression

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-memRFP-3xPax7gRNA

    Plasmid

    #224569

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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