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Showing: 1141 - 1160 of 1663 results

  1. pMCh-660

    Plasmid
    #92259
    Purpose
    Plasmid for expression of NHA-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells
    Depositor
    Witold Filipowicz, Marina Chekulaeva
    Article
    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1
    Insert
    TNRC6C CED (TNRC6C Human)
    Use
    Tags
    NHA
    Expression
    Mammalian
    Mutation
    C-terminal effector domain (CED): aa 1369-1690 wi…
    Promoter
    CMV
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    92259-plasmid-map-sequence-id-194509

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  2. pMCh-665

    Plasmid
    #92254
    Purpose
    Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs and mutated PAM2 motif in mammalian cells
    Depositor
    Witold Filipowicz, Marina Chekulaeva
    Article
    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1
    Insert
    TNRC6C CED (TNRC6C Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    C-terminal effector domain (CED): aa 1369-1690 wi…
    Promoter
    EF1A
    Available since
    Oct. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92254-plasmid-map-sequence-id-194411

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  5. eGFP-proα2(I)-L

    Plasmid
    #120160
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    120160-plasmid-map-sequence-id-233870

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  6. mApple-proα2(I)-L

    Plasmid
    #120161
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    120161-plasmid-map-sequence-id-233911

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  8. CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

    Plasmid
    #154196
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC1-A, sgRNA-BC1-C
    Use
    Lentiviral; Catch barcode activation
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and mU6
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154196-plasmid-map-sequence-id-308676

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  10. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

    Plasmid
    #190236
    Purpose
    AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase
    Depositor
    Hiroyuki Hioki
    Article
    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi
    Insert
    EGFP
    Use
    AAV
    Tags
    F2A, LDLR C-terminal sequence, mRFP1, myristoylat…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    190236-plasmid-map-sequence-id-381571

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  13. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid
    #154197
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Article
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC2-A, sgRNA-BC2-C
    Use
    Lentiviral; Catch barcode activation
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and mU6
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154197-plasmid-map-sequence-id-308682

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  14. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

    Plasmid
    #170240
    Purpose
    Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    MAP2K1 (MAP2K1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    S218D, S222D
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170240-plasmid-map-sequence-id-334554

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  19. eGFP-proα1(I)

    Plasmid
    #119843
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119843-plasmid-map-sequence-id-240348

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Showing: 1141 - 1160 of 1663 results