1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pcDNA3.1-Hyg-nCas9n

   Plasmid

   #217487

   Purpose

   Expression of nuclear nCas9n

   Depositor

   Bertrand Collet

   Article

   Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. P1K-HFL1-8flag-LEU

   Plasmid

   #207035

   Purpose

   Expression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Use

   Tags

   8xFLAG

   Expression

   Yeast

   Mutation

   Promoter

   pHFL1

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PCUP1-4V5-Pho8-Met

   Plasmid

   #207029

   Purpose

   Expression of 4V5-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   4xV5

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BS-Trp-PCUP1-4myc-Pho8

   Plasmid

   #207028

   Purpose

   Expression of 4Myc-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Pho8
   

   Use

   Tags

   4xMyc

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PCUP1-Vac8-GFP-Ura

   Plasmid

   #207027

   Purpose

   Expression of Vac8-GFP under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Vac8
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PCUP1-GFP-Vma13-Ura

   Plasmid

   #207026

   Purpose

   Expression of GFP-Vma13 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Vma13 ORF
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKERep

   Plasmid

   #211826

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. P1K-3HA-Atg8∆GR-MET15

   Plasmid

   #207033

   Purpose

   Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆GR

   Promoter

   pATG8

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PCUP1-mRuby3-Pho8-406

   Plasmid

   #207043

   Purpose

   Expression of mRuby3-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   PHO8
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKESa

    Plasmid

    #211828

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJR152

    Plasmid

    #196280

    Purpose

    Cassette used in dual sgRNA library generation

    Depositor

    Rebecca Voorhees

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Insert

    hU6-sgRNA-CR3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDGE1176

    Plasmid

    #202774

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDGE1177

    Plasmid

    #202775

    Purpose

    CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:TREX2-zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRA1EGFP

    Plasmid

    #193791

    Purpose

    Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PR promoter

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. UG75-PAH1-3HA

    Plasmid

    #184761

    Purpose

    PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    PAH1 (PAH1 Budding Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    PAH1

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. UG75-CDS1-3HA

    Plasmid

    #184760

    Purpose

    CDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    CDS1 (CDS1 Budding Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    CDS1

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. UG75-DGK1-3HA

    Plasmid

    #184759

    Purpose

    DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    DGK1 (DGK1 Budding Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    DGK1

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OM14-2EGFP-UG74-ter

    Plasmid

    #184758

    Purpose

    Marker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    OM14 (OM14 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SEC7-2EGFP-ter-NAT

    Plasmid

    #184764

    Purpose

    Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    SEC7 (SEC7 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. IRE1-2EGFP-ter-kanMX4

    Plasmid

    #184766

    Purpose

    Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    IRE1 (IRE1 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge