Addgene

We narrowed to 6,834 results for: 11

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ATF4 8: (SL-)5’ATF4.GFP

Plasmid

#21855

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pconst #3-RFP

Plasmid

#81077

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

Promoter

Constitutive Promoter - BBa_J23101

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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MTK1_018

Plasmid

#123681

Purpose

Encodes ConL8 as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L8

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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myc GbetaL mutant 5

Plasmid

#8479

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

G192D

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
mCherry-Supervillin-45

Plasmid

#55136

Purpose

Localization: Membrane/Actin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Supervillin

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
TALEN-CCR5L18-CtermCan

Plasmid

#51438

Purpose

Expresses TALEN targeting left CCR5A site (L18) with canonical C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) Can C-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5R18-CtermCan

Plasmid

#51439

Purpose

Expresses TALEN targeting right CCR5A site (R18) with canonical C-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) Can C-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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ATF4 19: (SL)RLuc.5’ATF4.GFP

Plasmid

#21865

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stem Loop upstream of ATF4

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG

Plasmid

#132684

Purpose

AAV plasmid encoding mouse PM20D2 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 2 (Pm20d2 Mouse)

Use

AAV

Tags

Flag

Expression

Mutation

Promoter

Chicken b-actin

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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MTK5_011

Plasmid

#123855

Purpose

Encodes ConR8 as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector R8

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pconst #2-RFP

Plasmid

#81076

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

Promoter

Constitutive Promoter- BBa_J23104

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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MTK2_014

Plasmid

#123706

Purpose

Encodes pUAS (miniCMV core) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pUAS (mini CMV)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLaNGo11-3

Plasmid

#126054

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

chloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)

Use

Binary

Tags

GFP1-10

Expression

Plant

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMiNGo11-1

Plasmid

#126056

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

mitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11

Use

Binary

Tags

GFP1-10

Expression

Plant

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4_004

Plasmid

#123813

Purpose

Encodes spacer sequence as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_018

Plasmid

#123710

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-mAV

Plasmid

#129472

Purpose

Mammalian expression of full-length mouse archvillin (Supervillin, isoform 2)

Depositor

Elizabeth Luna

Article

Oh et al J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-

Insert

Supervillin, isoform 2 (Svil Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1259

Plasmid

#44479

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, peel-1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pWay21-MKP-1CH2AB

Plasmid

#13475

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2AB (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-136

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
PYDonly-NLRP11.MYC

Plasmid

#131198

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

PYD domain of NLRP11 (NLRP11 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

contains only the pyrin domain (amino acids 1-128)

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ1200

Plasmid

#44485

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, PuroR, peel-1, MCS

Use

Multiple cloning site vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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1208 p3e-long etv2 3’UTR

Plasmid

#49010

Purpose

Zebrafish Long length etv2 3' UTR in gateway 3' Entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Tags

Expression

Mutation

Longest 3' UTR

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk3a2(2nd methione)

Plasmid

#15747

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2(2nd methione) (MAPK10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
TALEN-CCR5L18-NtermN1

Plasmid

#51444

Purpose

Expresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) N1 N-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K150Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
ATF4 9: (SL+)5’ATF4.GFP

Plasmid

#21856

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stem loop introduced at the 5' end of the mR…

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
myc GbetaL mutant 7

Plasmid

#8481

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

F320S

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
myc GbetaL mutant 2

Plasmid

#8478

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S72D

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pMCh-1016

Plasmid

#92255

Purpose

Plasmid for expression of HA-tagged human TNRC6C CED in mammalian cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

HA

Expression

Mammalian

Mutation

.C-terminal effector domain (CED): aa 1369-1690

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 C140S (#622)

Plasmid

#99947

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C140S

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCh-133

Plasmid

#92256

Purpose

Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

.C-terminal effector domain (CED): aa 1369-1690

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
myc GbetaL mutant 1

Plasmid

#8477

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

D42A

Promoter

Available since

May 11, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
myc GbetaL mutant 6

Plasmid

#8480

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

GbetaL (MLST8 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T208D

Promoter

Available since

May 11, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pRY1004

Plasmid

#21788

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Dig1-YFP (DIG1 A. victoria, Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

contains 5' 1000bp UTR (Dig1 promoter) Di…

Promoter

Available since

Sept. 18, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
RNAPII_CTD43-52

Plasmid

#98684

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

hepatds 43-52, residues 1888-1970

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD

Plasmid

#73306

Purpose

Expresses mutant human PUM1-HD

Depositor

Traci Hall

Article

Wang et al Nat Methods. 2009 Nov;6(11):825-30. doi:

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Use

Tags

intein

Expression

Bacterial

Mutation

Q867E-Q939E-C935S-Q1011E-C1007S

Promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS2_HindIII

Plasmid

#65666

Purpose

FLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII site

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS2 (RBPMS2 Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

introduced HindIII site (final goal to exchange F…

Promoter

CMV, 2x Tet

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pWay21-MKP-1DCH2B

Plasmid

#13471

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1DCH2B (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of MKP-1 CH2B; Delta 47-137

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
TALEN-CCR5R18-NtermN1

Plasmid

#51445

Purpose

Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) N1 N-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K150Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TALEN-CCR5L18-NtermN2

Plasmid

#51446

Purpose

Expresses TALEN targeting left CCR5A site (L18) with N2 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) N1 N-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K150Q and K153Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TALEN-CCR5R18-NtermN2

Plasmid

#51447

Purpose

Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) N2 N-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K150Q and K153Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFP121

Plasmid

#21670

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been modified, the E…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pRY1001

Plasmid

#21787

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

CFP-Ste12 (STE12 A. victoria, Budding Yeast)

Use

Tags

CFP

Expression

Yeast

Mutation

contains 1000 bp UTR (Ste12 promoter) upstream of…

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ1000

Plasmid

#44475

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, PuroR, peel-1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pWay21-MKP-1CH2A

Plasmid

#13473

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-46

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2B

Plasmid

#13474

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2B (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 47-136

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L16A

Plasmid

#13476

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L16A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 16 to Alanine

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L17A

Plasmid

#13477

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L17A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 17 to Alanine

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
Venus-rab11a

Plasmid

#234744

Purpose

Mammalian expression of recycling endosome marker Venus-rab11a

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-rab11a (RAB11A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-rab7a

Plasmid

#234743

Purpose

Mammalian expression of late endosome marker Venus-rab7a

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-rab7a (Rab7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry_EHMT1_full length

Plasmid

#228810

Purpose

Expression of mouse EHMT1

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (Ehmt1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

See Depositor Comments

Promoter

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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