Addgene

We narrowed to 2,243 results for: hells

Showing: 1711 - 1740 of 2243 results

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pUASt-FMW-attB-AthHen1

Plasmid

#104958

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila using somatic tissue Gal4 drivers

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Use

Tags

FLAG Myc

Expression

Insect

Mutation

Promoter

Hsp70 promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET28:LanIII

Plasmid

#225075

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIII)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanIV-Streptomyces

Plasmid

#226250

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Streptomyces)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyADO

Plasmid

#226248

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyO

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyAO

Plasmid

#226242

Purpose

Production of ApyA peptide modified by the cytochrome P450 enzyme ApyO

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyO

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyAD

Plasmid

#226241

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD

Use

Tags

Hexa-Histidine

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CB2R-GFP

Plasmid

#208919

Purpose

encodes the human cannabinoid receptor 2, tagged with GFP

Depositor

Christian Gruber

Article

Tomašević et al J Biol Chem. 2024 Jun;300(6):107330. doi

Insert

CNR2 (CNR2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

MCV

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-56

Plasmid

#225073

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-56)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2C

Plasmid

#225070

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2C)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2A

Plasmid

#225068

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2A)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-2B

Plasmid

#225069

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2B)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanIII-43

Plasmid

#225077

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIII-43)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-57

Plasmid

#225074

Purpose

Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanII-57)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanIII-6

Plasmid

#225076

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA(A) (LanIII-6)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2E

Plasmid

#225072

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2E)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-23

Plasmid

#225067

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-23)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII

Plasmid

#225066

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanII-2D

Plasmid

#225071

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2D)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHis-WzzE

Plasmid

#196661

Purpose

Expression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Use

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGST-WzzE

Plasmid

#196662

Purpose

Expression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Use

Tags

TEV-cleavable GST

Expression

Bacterial

Mutation

Valine insertion at position 2.

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHis-mCherry-WzzE

Plasmid

#196663

Purpose

Expression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Use

Tags

His and mCherry

Expression

Bacterial

Mutation

Valine insertion at position 2 of WzzE.

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPglL-strep-mCherry

Plasmid

#196664

Purpose

Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

PglL

Use

Tags

TEV-cleavable distrep tag II and mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPglL-strep-mClover3

Plasmid

#196665

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

PglL

Use

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWzyE-GST-distrepII

Plasmid

#196666

Purpose

Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

GST and TEV-cleavable di-strep tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWzyE-strep-mClover3

Plasmid

#196668

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-Halo-H2DBD

Plasmid

#200206

Purpose

Expression of truncated HSF2 (DBD)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HA-Halo-H2DBD (Hsf2 Mouse)

Use

Tags

Halo; HA

Expression

Mammalian

Mutation

HSF2 (1-366; 15 bp linker; 972-1017)

Promoter

EF_1alpha

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
NLS-Halo-HSF2

Plasmid

#200198

Purpose

Expression of full length HSF2 with SV40 NLS

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X SV40 NLS-Halo-HSF2 (Hsf2 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-H2DBD-HR

Plasmid

#200199

Purpose

Expression of truncated HSF2 (DBD and HR)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-H2DBD-HR (Hsf2 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

HSF2 (1-627)

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-H2DBD

Plasmid

#200200

Purpose

Expression of truncated HSF2 (DBD)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-H2DBD (Hsf2 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

HSF2 (1-366; 15 bp linker; 972-1017)

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-Halo-HSF2

Plasmid

#200202

Purpose

Expression of full length HSF2

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HA-Halo-HSF2 (Hsf2 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge