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pLenti CMV RasV12 Neo (w108-1)

Plasmid

#22259

Depositor

Eric Campeau

Insert

HRAS G12V (HRAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

G12V mutant

Promoter

Available since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgControl

Plasmid

#125836

Purpose

KO control

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

Control

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_ACh3.0

Plasmid

#121922

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Has Service

AAV9

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC3A2

Plasmid

#132213

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC3A2 (SLC3A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-FLAG-LKB1

Plasmid

#8590

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec23A

Plasmid

#66609

Purpose

GFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-ATG9A

Plasmid

#198529

Purpose

Expression of ATG9A-GFP fusion protein in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

ATG9A (ATG9A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations in codons 4 and 775

Promoter

CMV

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-S33Y Beta-catenin

Plasmid

#19286

Purpose

Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S33Y (presumptive GSK3β phosphorylation site)

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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HLA_A0101

Plasmid

#165009

Purpose

Expression of HLA-A*01:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-A*01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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HA-VHL-pRc/CMV

Plasmid

#19999

Depositor

William Kaelin

Article

Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Promoter

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO eNpHR 3.0-EYFP

Plasmid

#26966

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

ER export at 3' end of gene. Trafficking sig…

Promoter

EF1alpha

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pENN226_CRISPRcharm

Plasmid

#220834

Purpose

CRISPRcharm expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CCR5

Plasmid

#98943

Purpose

Mammalian expression plasmid for untagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Use

Tags

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Hs.cargo(Cre)

Plasmid

#174863

Purpose

Expresses human Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by HsPeg10 UTRs

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF0731

Plasmid

#142201

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PROX1 (PROX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

Plasmid

#105842

Purpose

Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
Optimized mApple
rtTA3G
SBP Delta-LNGFR

Use

CRISPR and TALEN

Tags

Expression

Mutation

Promoter

CAG, EF-1alpha, and TRE3G

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp9-His

Plasmid

#11829

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

Insert

Caspase 9 (CASP9 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
PH-PLCD1-GFP

Plasmid

#51407

Purpose

Is a biosensor for PI(4,5)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A3

Plasmid

#135505

Purpose

Mammalian expression of myc-tagged HLA-A*03:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*03:01 (HLA-A Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA-mSin1(1.1)-HA

Plasmid

#73388

Purpose

Expresses mSin-HA

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

mSin1 transcript variant protein isoform 1 (MAPKAP1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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