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Plasmid#24909DepositorInsertfishRab8B
UseGateway entry vectorTagsExpressionMutationChanged Threonine 22 to AsparaginePromoterAvailable sinceJune 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
ZE9 tiggywinkle hedgehog pT7TS
Plasmid#17058DepositorInserttiggywinkle hedgehog (shhb Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
ZP61 Isthmin
Plasmid#16881DepositorInsertisthmin (ism1 Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
M25 NacMS>Nac
Plasmid#17175DepositorInsertnacre promoter driving nacre cDNA (mitfa Zebrafish)
UseTagsExpressionMutationAll three Tcf/Lef sites are mutatedPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pNDL-54
Plasmid#161715PurposeIn vitro transcription construct for production of membrane acvr1b-Cry2 mRNADepositorInsertMyr-acvr1b-Cry2 (acvr1ba Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNDL-60
Plasmid#161720PurposeIn vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNADepositorInsertcytosolic acvr2b-CIB1N (acvr2b Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2
Plasmid#218206PurposeZebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert+15.2prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218207PurposeZebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_1
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2
Plasmid#218208PurposeZebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_2
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2_-87prox1a:basEGFP_Acry:GFP
Plasmid#218213PurposeZebrafish -87prox1a enhancer reporter in the lymphatics at 5 dpf. Acry_GFP as a control in the lensDepositorInsert-87prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218205PurposeZebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1
Plasmid#198240PurposeFor I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sitesDepositorInsertsUseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKE13-ISceI-fabp10a:CreERT2
Plasmid#198242PurposeFor I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinaseDepositorInsertsUseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDR274-gsc2-sgRNA
Plasmid#184818PurposeUsed to synthesize gRNA targeting exon 2 of the gsc2 geneDepositorArticleInsertgsc2-sgRNA
UseCRISPR; Template for sgrna synthesisTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-QF2-he1.1:YFP-rln3a-HA
Plasmid#184820PurposeModified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.DepositorArticleInsertrln3a-HA
UseCRISPR; For integration of qf2 by homology-direct…TagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TOPO-nmba
Plasmid#184821PurposeUsed to synthesize RNA probe for in situ hybridization for nmbaDepositorArticleInsertnmba (nmba Zebrafish)
UseTemplate for synthesis of nmba probeTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TOPO-nmbb
Plasmid#184822PurposeUsed to synthesize RNA probe for in situ hybridization for nmbbDepositorArticleInsertnmbb (nmbb Zebrafish)
UseTemplate for synthesis of nmbb probeTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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GBH-2aG4-insBFP.1
Plasmid#188025PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker insulin BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; insulin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-insGFP.1
Plasmid#188028PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker insulin GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; insulin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only