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non-targeting control gRNA (BRDN0001146327)

Plasmid

#80188

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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WNK1 gRNA (BRDN0001146391)

Plasmid

#75622

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK1 (WNK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK1 gRNA (BRDN0001147437)

Plasmid

#75623

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK1 (WNK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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WNK1 gRNA (BRDN0001148217)

Plasmid

#75624

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK1 (WNK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shDUSP19 puro

Plasmid

#87796

Purpose

Expresses an inducible short hairpin targeting human DUSP19 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shDUSP19 (DUSP19 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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RAB11FIP5 gRNA (BRDN0001148210)

Plasmid

#76185

Purpose

3rd generation lentiviral gRNA plasmid targeting human RAB11FIP5

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RAB11FIP5 (RAB11FIP5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RAB11FIP5 gRNA (BRDN0001148953)

Plasmid

#76187

Purpose

3rd generation lentiviral gRNA plasmid targeting human RAB11FIP5

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RAB11FIP5 (RAB11FIP5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PI4KB gRNA (BRDN0001162483)

Plasmid

#76542

Purpose

3rd generation lentiviral gRNA plasmid targeting human PI4KB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PI4KB (PI4KB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PI4KB gRNA (BRDN0001145642)

Plasmid

#76543

Purpose

3rd generation lentiviral gRNA plasmid targeting human PI4KB

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PI4KB (PI4KB Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK16 gRNA (BRDN0001147125)

Plasmid

#76903

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK16

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK16 (CDK16 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GAK gRNA (BRDN0001146687)

Plasmid

#76470

Purpose

3rd generation lentiviral gRNA plasmid targeting human GAK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GAK (GAK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAD2L1BP F1.1 gRNA

Plasmid

#90744

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1BP (Guide Designation F1.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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MAD2L1BP F2.1 gRNA

Plasmid

#90745

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1BP (Guide Designation F2.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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TGFBR3 gRNA (BRDN0001144841)

Plasmid

#76292

Purpose

3rd generation lentiviral gRNA plasmid targeting human TGFBR3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TGFBR3 (TGFBR3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DCLK2 gRNA (BRDN0001162199)

Plasmid

#75701

Purpose

3rd generation lentiviral gRNA plasmid targeting human DCLK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DCLK2 (DCLK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfRab25_4

Plasmid

#26712

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Canine Rab25 RNAi.

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
SPHK1 gRNA (BRDN0001147454)

Plasmid

#76813

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK1 (SPHK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SPHK1 gRNA (BRDN0001147534)

Plasmid

#76814

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK1 (SPHK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ (M)-HT-Cbx7-Delta-ATL

Plasmid

#82523

Purpose

Lentivirus vector. Expresses mutant Halotag-Cbx7-delta-ATL fusion proteins in mammalian cells. Cbx7deletion of amino acids 66–83.

Depositor

Xiaojun Ren

Insert

Chromobox Homolog 7 (CBX7 Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

deletion of amino acids 66–83

Promoter

CMV

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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TYK2 gRNA (BRDN0001147182)

Plasmid

#77247

Purpose

3rd generation lentiviral gRNA plasmid targeting human TYK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TYK2 (TYK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge