Showing: 11521 - 11540 of 16040 results
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Plasmid#64771PurposeMammalian protein expression of flag-tagged Med25DepositorInsertMed25 (MED25 Human)
UseTagsFlagExpressionMammalianMutationPromoterpCMVAvailabilityAcademic Institutions and Nonprofits only -
Flag_HsIRE1a_pBabePuro
Plasmid#54337PurposeN-terminally FLAG tagged human IRE1a in retroviral pBabe puro vectorDepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationThe endogenous IRE1a signal sequence (aa1-18) was…PromoterAvailabilityAcademic Institutions and Nonprofits only -
Flag_HsIRE1a_deltaP29_D408_pBabePuro
Plasmid#58422Purposeretroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408DepositorInsertIRE1a (ERN1 Human)
UseRetroviralTagsFLAG and preprotrypsin signal sequenceExpressionMammalianMutationDeleted amino acids P29 to D408PromoterAvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344A-mCerulean
Plasmid#69580PurposeExpresses p53-L344A mutant tagged with mCeruleanDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344P-mCerulean
Plasmid#69581PurposeExpresses p53-L344P mutant tagged with mCeruleanDepositorInsertp53-L344P (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationp53 L344P mutation that prevents oliogomerization…PromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC
Plasmid#69585PurposeExpresses mutated p53-L344A tagged with mKate2 and split C-term mVenusDepositorInsertp53-L344A (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - C termExpressionMammalianMutationp53 L344A mutation that prevents tetramerization …PromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
KCC2-pHext
Plasmid#104076PurposeTo visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
UseTagspHluorine tag inserted in the second putative tra…ExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterUbCAvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX11-Flag-WT
Plasmid#120727PurposeExpression of a Flag-tagged version of DDX11 WT in mammalian cellsDepositorInsertDDX11 (DDX11 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pKK-MBP-TEV
Plasmid#105771PurposeExpression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).DepositorTypeEmpty backboneUseFlp-in competentTagsMBP-TEVExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-MBP
Plasmid#105787PurposeExpression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-MBPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA GASTM-3-RVG-10-Lamp2b-HA
Plasmid#71295PurposeEncodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
UseTagsGASTM mutated glycosylation motif, HA, Lamp2 sig…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCR3-FLAG-Gephyrin E
Plasmid#68819PurposeFlag tagged mammalian expression of E domainDepositorInsertGephyrin (Gphn Rat)
UseTags3xFLAGExpressionMammalianMutationinternal EcoRI site has silent mutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-Seipin-miniIAA7-mCherry
Plasmid#129720PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged SeipinDepositorInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-mCherryExpressionMammalianMutationsilent mutationsPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I
Plasmid#129721PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged SeipinDepositorInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-mScarlet-IExpressionMammalianMutationsilent mutationsPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-Seipin-miniIAA7-3XFlag
Plasmid#129722PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged SeipinDepositorInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-3XFlagExpressionMammalianMutationsilent mutationsPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pSLIK_neo_3xflag_CHEK2
Plasmid#136536PurposeLentiviral expression vector for an inducible 3xflag-tagged CHEK2DepositorInsertCHEK2 (CHEK2 Human)
UseLentiviral; Destinatioin vector for gateway cloni…Tags3x FLAGExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBABE_hygro_mRFP1_NRF2(C506S, L4A)
Plasmid#136580Purposemammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)DepositorInsertNRF2 (C506S, Leuzip mut) (NFE2L2 Human)
UseRetroviralTagsN-mRFP1-FLAGExpressionMammalianMutationThe C506S mutation (C513S in the actual sequence …PromoterAvailabilityAcademic Institutions and Nonprofits only -
GFP-FBXL11
Plasmid#126543PurposeExpresses GFP-tagged FBXL11 in mammalian cellsDepositorInsertFBXL11 (KDM2A Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterLTRAvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT POLI
Plasmid#131228PurposeExpression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationCoding sequence has been codon optimised for expr…PromoterCMV enhancer + CMV promoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_UBM1*2* POLI
Plasmid#131232PurposeMammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domainsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains inactivating mutations in both ubiquitin…PromoterCMV enhancer + CMV promoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 11521 - 11540 of 16040 results