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Plasmid#152993PurposeC-to-T base editorDepositorInsertBE4-nSpCas9 CP1028-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-HNHx-ABEmax7.10HD-BlastR
Plasmid#176477PurposeA-to-G base editor with alternative targeting scopeDepositorInsertHNHx-ABEmax7.10HD
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-AasgRNA
Plasmid#121954PurposeMammalian expression, Genome editing, gRNA scaffoldDepositorInsertAaCas12b single chimeric gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0508
Plasmid#91014PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9
UseCRISPRTagsExpressionMutationPromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE8
Plasmid#79463PurposesgRNA shuttle vector; module 2EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
e-ciCas9_pcDNA5
Plasmid#100553PurposeExpresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.DepositorInserte-ciCas9
UseCRISPR; Frt, flp-inTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP
Plasmid#84256PurposeExpresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent markerDepositorInsertSa sgCXCR4-3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCF730
Plasmid#121668PurposeEF1a-ProCas9-Flavi-P2A-PuroDepositorInsertCas9(C)
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1111
Plasmid#91032PurposeModule A, Promoter: OsAct1, Gene: TaCas9, Terminator: HSPDepositorInsertTaCas9
UseCRISPRTagsExpressionMutationPromoterOsAct1Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFP
Plasmid#84255PurposeExpresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent markerDepositorInsertSa sgCXCR4-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA-mCtnnb1
Plasmid#84542PurposemCtnnb1 gRNA expressing vectorDepositorInsertmCtnnb1 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1511
Plasmid#91037PurposeModule A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSPDepositorInsertCsy4-P2A-TaCas9
UseCRISPRTagsExpressionMutationPromoterOsAct1Available sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE6
Plasmid#79476PurposesgRNA shuttle vector; module 1EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-U6-sgRNA-CMV-puro
Plasmid#169450Purposebackbone for sgRNA expressionDepositorInsertsgRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCas9-Nours
Plasmid#107733PurposeEscherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiaeDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionYeastMutationHuman optimizedPromoterTEF1Available sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0908
Plasmid#91027PurposeModule A, Promoter: EC1.2, Gene: TREX2-P2A-AtCas9, Terminator: HSPDepositorInsertTREX2-P2A-AtCas9
UseCRISPRTagsExpressionMutationPromoterEC1.2Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV Gsk3 sgRNA/GFP
Plasmid#112733PurposeGsk3b targeting gRNA cloned into px552 (SpGuide) plasmid.DepositorInsertGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE12
Plasmid#79469PurposesgRNA shuttle vector; module 5DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE13
Plasmid#79471PurposesgRNA shuttle vector; module 6DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE14
Plasmid#79473PurposesgRNA shuttle vector; module 7DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only