Showing: 11521 - 11540 of 18100 results
-
Plasmid#138155PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-NG
Plasmid#138159PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10A + …PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
dead Sa Cas9
Plasmid#138162PurposeFor orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)DepositorInsertdSaCas9
UseTagsNLSExpressionMammalianMutationD10A + N580APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-(empty)-TAG
Plasmid#138209PurposeTo facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' regionDepositorTypeEmpty backboneUseT7 transcriptionTagsTAG sequenceExpressionMammalianMutationPromoterCMV Promoter, T7 PromoterAvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA
Plasmid#138271PurposeDual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)DepositorInsertsgBG, sgTS
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEF-BFP
Plasmid#138272PurposeExpression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editorDepositorInsertBFP H66 PAM
UseCRISPRTagsExpressionMammalianMutationH66, PAM motifPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
-
pCW57.1 Lysosomal-METRIQ
Plasmid#135401PurposeExpresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activityDepositorInsertDNAse II alpha (DNASE2 Human)
UseLentiviralTagsT2A, mCherry, and sfGFPExpressionMammalianMutationSingle-nucleotide polymorphism C432TPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP TFEB-sfGFP
Plasmid#135402PurposeExpresses TFEB-sfGFPDepositorInsertTFEB (TFEB Human)
UseRetroviralTagssfGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMH0006
Plasmid#135448PurposeHuman expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.DepositorInsertdCas9-BFP-KRAB
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterEF1AAvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5
Plasmid#135455PurposeBacterial expression of uTEV1Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, S153N mutation…Promotertac promoterAvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5
Plasmid#135456PurposeBacterial expression of uTEV2Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, T30A/S153N mut…Promotertac promoterAvailabilityAcademic Institutions and Nonprofits only -
UAS-mCherry
Plasmid#135457PurposeMammalian mCherry reporter to read out FLARE and SPARK activationDepositorInsertmCherry
UseTagsmCherryExpressionMammalianMutationPromoterUASAvailabilityAcademic Institutions and Nonprofits only -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A2
Plasmid#135504PurposeMammalian expression of myc-tagged HLA-A*02:01DepositorInsertHLA-A*02:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-G3
Plasmid#135521PurposeEngineered form of fibroblast growth factor 2 with improved thermostabilityDepositorInsertfibroblast growth factor 2 (FGF2 Human)
UseTags6x His tag and Agg Thrombin TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailabilityAcademic Institutions and Nonprofits only -
pXT128_TwinStrep-SUMO-ShCas12k
Plasmid#135524PurposeProtein expression construct for ShCast12kDepositorInsertShCas12k
UseTagsTwinStrep-SUMOExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pXT129_TwinStrep-SUMO-ShTnsB
Plasmid#135525PurposeProtein expression construct for ShTnsBDepositorInsertShTnsB
UseTagsTwinStrep-SUMOExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pXT130_TwinStrep-SUMO-ShTnsC
Plasmid#135526PurposeProtein expression construct for ShTnsCDepositorInsertShTnsC
UseTagsTwinStrep-SUMOExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pXT131_TwinStrep-SUMO-ShTniQ
Plasmid#135527PurposeProtein expression construct for ShTniQDepositorInsertShTniQ
UseTagsTwinStrep-SUMOExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 11521 - 11540 of 18100 results