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Addgene
Showing: 11501 - 11520 of 16040 results
  1. 1BR9-AtWUSSTOP-Δα3

    Plasmid
    #202056
    Purpose
    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.
    Depositor
    Insert
    AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
    Use
    Tags
    6xHis and GFP11
    Expression
    Bacterial
    Mutation
    third helix of homeodomain deleted (AA82-102)
    Promoter
    T7 Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pLJM1-B-GRAM-H-NeoR

    Plasmid
    #211709
    Purpose
    Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H)
    Depositor
    Insert
    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Lentiviral
    Tags
    ddFP-B3
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Leucine
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  3. pLJM1-B-GRAM-W-NeoR

    Plasmid
    #211710
    Purpose
    Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W)
    Depositor
    Insert
    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Lentiviral
    Tags
    ddFP-B3
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Tryptophan
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  4. p426_Cas9_gRNA-R-ARS805a

    Plasmid
    #87408
    Purpose
    p426_Cas9_gRNA-ARS805a without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Availability
    Academic Institutions and Nonprofits only
  5. pTRIPZ (M)-HT-Cbx6

    Plasmid
    #82514
    Purpose
    Lentivirus vector. Expresses Halotag-Cbx6 fusion proteins in mammalian cells.
    Depositor
    Insert
    Chromobox Homolog 6 (CBX6 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Availability
    Academic Institutions and Nonprofits only
  6. pTRIPZ (M)-HT-Cbx7

    Plasmid
    #82515
    Purpose
    Lentivirus vector. Expresses Halotag-Cbx7 fusion proteins in mammalian cells.
    Depositor
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Availability
    Academic Institutions and Nonprofits only
  7. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid
    #140082
    Purpose
    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter
    Depositor
    Insert
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    Use
    In vitro transcription (mrna synthesis)
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA3.2-GJA1-20K-V5

    Plasmid
    #49859
    Purpose
    Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag
    Depositor
    Insert
    GJA1-20k (GJA1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    GJA1-20k fragment corresponds to residues 213-382…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 11501 - 11520 of 16040 results