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Plasmid#172578PurposeFor generation of FlpIn lines with untagged MACROD1DepositorInsertMACROD1 (MACROD1 Human)
UseTagsnoneExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/MACROD2
Plasmid#172579PurposeFor generation of FlpIn lines with untagged MACROD2DepositorInsertMACROD2 (MACROD2 Human)
UseTagsnoneExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN S473A 513A
Plasmid#175763PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN S473A 513ADepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationS473A S513APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN D474N
Plasmid#175762PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN D474NDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationD474NPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_VSX1
Plasmid#167808PurposeExpresses VSX1 in mammalian cellsDepositorInsertVSX1 (VSX1 Human)
UseTagsMACExpressionMammalianMutationno mutationsPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
SITS-ccdb
Plasmid#141067PurposeMultisite gateway destination vector for cell free expression (takes two cassettes). Parton lab clone JBODepositorInsertccdb
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-ccdb-EGFP
Plasmid#118752PurposeMultisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEXDepositorInsertccdb
UseTagsEGFPExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-mCherry-ccdb
Plasmid#118629PurposeMultisite gateway destination vector for 5' tagging with mCherry for cell free expression. Parton lab clone FKZDepositorInsertccdb
UseTagsmCherryExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SITS-EGFP-ccdb
Plasmid#118628PurposeMultisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZDepositorInsertccdb
UseTagsEGFPExpressionMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ash1
Plasmid#169711PurposeBacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)DepositorInsertAsh1 (ASH1 Budding Yeast)
UseTags6xHisExpressionBacterialMutationTruncation including encoding amino acids 420-500PromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-GgVCL/*TBM
Plasmid#162790PurposeMUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cellsDepositorArticleInsertMutated Gallus gallus vinculin (G454V) (VCL Chicken)
UseTagsIRES-GFPExpressionBacterial and MammalianMutationchanged Gly 454 to ValPromoterbeta actinAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-Cas9
Plasmid#120352PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-KRAB-dCas9
Plasmid#120355PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBA cMYC-BPC4
Plasmid#118668PurposePlant overexpressionDepositorInsertBPC4 (BPC4 Mustard Weed)
UseTagsMycExpressionPlantMutationPromoterCaMV35SAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLENTI CMV GFP-TREX1 G306A BLAST
Plasmid#164241Purposelentiviral plasmid for GFP-TREX1 G306ADepositorInsertTREX1 (TREX1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationG306A point mutationPromoterCMVAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnma1a
Plasmid#164951PurposeZebrafish kcnma1a gene CDSDepositorInsertkcnma1a (kcnma1a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_2pabPAC(Y72L)
Plasmid#158970PurposeA negative control for AAV-mediated 2P-activatable bPACDepositorInsert2pabPACY72L
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA7
Plasmid#158485PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA15
Plasmid#158493PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, no STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA8
Plasmid#158486PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 L17E/D481V with STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationL17E, D481V, STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only