Addgene

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pMOD_A0301

Plasmid

#91005

Purpose

Module A, Promoter: 35S, Gene: AtCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_H840A nickase

Use

CRISPR

Tags

Expression

Mutation

H840A

Promoter

35S

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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TAL3178

Plasmid

#41352

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-sox2-Left (sox2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
SlutrCas9

Plasmid

#163795

Purpose

Expresses SlutrCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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ShaCas9

Plasmid

#163794

Purpose

Expresses ShaCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pOVC1_ScPH1-S_X

Plasmid

#131184

Purpose

Expresses ScPH1-S (Synechosystis PCC6803) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

ScPH1-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-2xFLAG-Atoh1ER

Plasmid

#101736

Purpose

Expresses mouse Atoh1 fused to ER

Depositor

Albert Edge

Article

Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

Insert

Atoh1 (Atoh1 Mouse)

Use

Tags

2x FLAG and ER

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0040

Plasmid

#117607

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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4xHRE_MLP-sfGFP-CMV_dsRed Exp

Plasmid

#117403

Purpose

sfGFP expressed from hypoxia-inducible MLP promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pZF-BCB-PDZ

Plasmid

#127478

Purpose

coding sequence for synthetic zinc finger ZF-BCB-PDZ

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain

Use

Cell-free transcription–translation

Tags

alpha 1-syntrophin PDZ domain

Expression

Mutation

Promoter

T7

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAFM-NbrWT

Plasmid

#40830

Purpose

Expresses Flag, Myc tagged Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr (Nbr Fly)

Use

Tags

3xFLAG and 6xMyc

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101115

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to LPAR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

LPAR1-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-6823

Plasmid

#84986

Purpose

For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-PMKco-Mkco

Use

Tags

Expression

Bacterial

Mutation

Promoter

LacUV5

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-6833

Plasmid

#84983

Purpose

Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

NudB-PMD

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Flag Snail 4SE

Plasmid

#16223

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

4SE: S108E, S112E, S116E, S120E

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
GFP Snail 2A4E

Plasmid

#16231

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

2A4E: S97A, S101A, S108E, S112E, S116E, S120E

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
YFP(1-158)-STOP

Plasmid

#66107

Purpose

This vector attaches YFP(1-158) to the C-terminus of a protein

Depositor

Catherine Berlot

Article

Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-3

Insert

YFP(1-158)STOP

Use

Tags

Expression

Mammalian

Mutation

Gln69 was changed to Met.

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Ro2

Plasmid

#24503

Depositor

Bruce Conklin

Article

Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(

Insert

RASSL Ro2 (OPRK1 Human)

Use

Tags

FLAG and NLS from prolactin

Expression

Mammalian

Mutation

mutations in extracellular loops 2 (see comments …

Promoter

Available since

June 16, 2010

Availability

Academic Institutions and Nonprofits only
pMOD_A3910

Plasmid

#91054

Purpose

Module A, Promoter: ZmUbi, Gene: dTaCas9-VPR, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

dTaCas9-VPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

ZmUbi

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVscCBPITuDlet-7Gpluc

Plasmid

#35652

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD let-7

Use

AAV

Tags

Expression

Mutation

Promoter

U6

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101113

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to CXCR4

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

CXCR4-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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