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pHAGE2-TetOminiCMV-OSK

Plasmid

#136552

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2-T2A-Klf4 (Klf4, Sox2, Pou5f1 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV:DsRed(FRT)GFP

Plasmid

#31128

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

DsRed(FRT)GFP

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-ZFN-AAVS1

Plasmid

#89707

Purpose

Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

Depositor

Dmitriy Mazurov

Article

Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

Insert

ZFN-AAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_MITF_P2A_Hygro_Barcode

Plasmid

#120460

Purpose

Barcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MITF (MITF Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-Citroff1

Plasmid

#134304

Purpose

Mammalian expression of inverse-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1

Plasmid

#36412

Depositor

Benjamin Glick

Type

Empty backbone

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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Ube1/PET21d

Plasmid

#34965

Depositor

Cynthia Wolberger

Article

Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

Insert

Ube1 (UBA1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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HA-APP-mGFP

Plasmid

#196696

Purpose

Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Use

Tags

HA and mGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEJS1099: Dual-sgRNA.Design 4

Plasmid

#159537

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 with two guide RNA cassettes

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Mutation

Promoter

U1a

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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