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IL2-mCherry-AHA740D, E758K in pmCherryN1

Plasmid

#187285

Purpose

Express IL2-AH A740D, E758K-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH A740D, E758K

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-R133C

Plasmid

#179409

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R133C)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(R133C) (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R133C

Promoter

CAGGS

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK9c_1 (inducible)

Plasmid

#189957

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK10c_2 (inducible)

Plasmid

#189956

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_2 (inducible)

Plasmid

#189954

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_1 (inducible)

Plasmid

#189953

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK9c_2 (inducible)

Plasmid

#189958

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-T158M

Plasmid

#179410

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(T158M)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(T158M) (Mecp2 Mouse)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD T158M

Promoter

CAGGS

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCSmChnERT(#99)

Plasmid

#184063

Purpose

cyclofen-inducible mCherry nuclear relocation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

Insert

mCherry-nls-ERT2

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet1-LanCL2-H264A-C-Ter-His

Plasmid

#154187

Purpose

To express LanCL2-H264A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

His264->Ala264

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pETDuet1-LanCL2-C321A/C367A-C-Ter-His

Plasmid

#154188

Purpose

To express LanCL2-C321A/C367A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Cys321->Ala321; Cys367->Ala367

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b-LanCL1-H219A

Plasmid

#154190

Purpose

To express LanCL1-H219A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL1

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

His219->Ala219

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b-LanCL1-C322A

Plasmid

#154191

Purpose

To express LanCL1-C322A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL1

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Cys322->Ala322

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-CREM

Plasmid

#178442

Purpose

doxycycline-inducible expression of Human CREM in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

CREM (CREM Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV hCgrrf1-V5

Plasmid

#175129

Purpose

Lentiviral expression of human CGRRF1-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

CGRRF1 (CGRRF1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLV mCgrrf1-V5

Plasmid

#175128

Purpose

Lentiviral expression of mouse Cgrrf1-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Cgrrf1 (Cgrrf1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-Truncated-Kir3.2-StreptagII

Plasmid

#177263

Purpose

Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

Depositor

Arthur Laganowsky

Article

Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

Insert

G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)

Use

Tags

StreptagII

Expression

Insect

Mutation

Residues 49-378

Promoter

polyhedrin promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn-Lrrc23-FLAG

Plasmid

#179921

Purpose

Vector backbone. pClgn-promoter driven expression.

Depositor

Masahito Ikawa

Article

Zhang et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

Lrrc23 (Lrrc23 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Stop codon removed

Promoter

Calmegin

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn-Lrrc23-1D4

Plasmid

#179922

Purpose

Vector backbone. pClgn-promoter driven expression.

Depositor

Masahito Ikawa

Article

Zhang et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

Lrrc23 (Lrrc23 Mouse)

Use

Tags

1D4

Expression

Mammalian

Mutation

Stop codon removed

Promoter

Calmegin

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-IFITM1

Plasmid

#172417

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Interferon-induced transmembrane protein 1 (CD antigen CD225) (IFITM1 Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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