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Rosa26_gRNA

Plasmid

#196138

Purpose

gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Rosa26 gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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AAVS1_gRNA

Plasmid

#196139

Purpose

gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

AAVS1 gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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sgNS-Hygromycin

Plasmid

#196198

Purpose

control

Depositor

Jianjun Chen

Insert

sgNS

Use

Lentiviral

Tags

Expression

Mutation

NO

Promoter

Availability

Academic Institutions and Nonprofits only

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sgMETTL16-1-Hygromycin

Plasmid

#196199

Purpose

knockout METTL16

Depositor

Jianjun Chen

Insert

sgMETTL16-1 (METTL16 Human)

Use

Lentiviral

Tags

Expression

Mutation

NO

Promoter

Availability

Academic Institutions and Nonprofits only

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sgMETTL16-2-Hygromycin

Plasmid

#196200

Purpose

knockout METTL17

Depositor

Jianjun Chen

Insert

sgMETTL16-2 (METTL16 Human)

Use

Lentiviral

Tags

Expression

Mutation

NO

Promoter

Availability

Academic Institutions and Nonprofits only

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pmiRNA1-OE-METTL16 F187G-sgMETTL16-MUT1

Plasmid

#196201

Purpose

overexpress METTL16

Depositor

Jianjun Chen

Insert

METTL16 F187G (METTL16 Human)

Use

Lentiviral

Tags

Expression

Mutation

F187G

Promoter

Availability

Academic Institutions and Nonprofits only

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pmiRNA1-OE-METTL16-sgMETTL16-MUT2

Plasmid

#196202

Purpose

overexpress METTL16

Depositor

Jianjun Chen

Insert

Synonymous mutation of METTL16 ORF (METTL16 Human)

Use

Lentiviral

Tags

Expression

Mutation

Synonymous mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Psin4-BCAT1

Plasmid

#196203

Purpose

overexpress BCAT1

Depositor

Jianjun Chen

Insert

BCAT1 (BCAT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

NO

Promoter

Availability

Academic Institutions and Nonprofits only

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PCDH-BCAT2

Plasmid

#196204

Purpose

overexpress BCAT2

Depositor

Jianjun Chen

Insert

BCAT2 (BCAT2 Human)

Use

Lentiviral

Tags

Expression

Mutation

NO

Promoter

Availability

Academic Institutions and Nonprofits only

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pmiRNA1-OE-METTL16 PP185/186AA-sgMETTL16-MUT1

Plasmid

#196205

Purpose

overexpress mutant METTL16

Depositor

Jianjun Chen

Insert

METTL16 PP185/186AA (METTL16 Human)

Use

Lentiviral

Tags

Expression

Mutation

PP185/186AA

Promoter

Availability

Academic Institutions and Nonprofits only

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pmiRNA1-OE-METTL16-sgMETTL16-MUT1

Plasmid

#196206

Purpose

overexpress mutant METTL16

Depositor

Jianjun Chen

Insert

Synonymous mutation of METTL16 ORF (METTL16 Human)

Use

Lentiviral

Tags

Expression

Mutation

Synonymous mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PCDH-METTL16-3*Flag

Plasmid

#196207

Purpose

overexpress METTL16

Depositor

Jianjun Chen

Insert

WT METTL16 (METTL16 Human)

Use

Lentiviral

Tags

Expression

Mutation

NO

Promoter

Availability

Academic Institutions and Nonprofits only

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Lenti-U6-sgMettl3

Plasmid

#196263

Purpose

Expresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoter

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Mettl3 sgRNA (Mettl3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pJR152

Plasmid

#196280

Purpose

Cassette used in dual sgRNA library generation

Depositor

Rebecca Voorhees

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Insert

hU6-sgRNA-CR3

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sp-HPV-TM(ICAM)

Plasmid

#196510

Purpose

Express HPV antigen epitope with ICAM transmembrane domain

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

a HPV epitope with CD8a stalk linker and ICAM TM domain

Use

Lentiviral

Tags

3xflag tag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-p5-b2m-HLA-A0101

Plasmid

#196512

Purpose

Express single-chain peptide-MHC (CMV p5 epitope on HLA-A0101)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a CMV peptide, b2m and HLA A0101

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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plentiCMV10x-sct-pp65-b2m-HLA-A0201

Plasmid

#196513

Purpose

Express single-chain pMHC with sequencing tag for 10x genomics

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C, 10xgenomics compatible tag

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1 sgTollip

Plasmid

#196546

Purpose

Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Lepr peak reporter-eGFP

Plasmid

#196584

Purpose

Cis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

eGFP

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Availability

Academic Institutions and Nonprofits only

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