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pcDNA3.1-H2-18 Light chain

Plasmid

#216308

Purpose

Expresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

H2-18 Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ARL16-His6

Plasmid

#216484

Purpose

Used for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tag

Depositor

Richard Kahn

Insert

ADP-ribosylation factor like 16 (ARL16 Human)

Use

Tags

includes GSGSGSHHHHHH (His6) tag at C-terminus

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-M

Plasmid

#215813

Purpose

Expresses SARS-CoV-2 Membrane protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Membrane (M )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-E

Plasmid

#215814

Purpose

Expresses SARS-CoV-2 Envelope protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Envelope (E )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hACE2-LgBiT

Plasmid

#215837

Purpose

Expresses hACE2 receptor with LgBiT tag in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

hACE2-LgBiT (ACE2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG/HA-DCP1

Plasmid

#215843

Purpose

Expression of FLAG/HA-DCP1

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human DCP1 (DCP1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG/HA-p54

Plasmid

#215844

Purpose

Expression of FLAG/HA-p54

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human p54

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-nLuc

Plasmid

#212611

Purpose

For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-nLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrasnatG12D

Plasmid

#206842

Purpose

To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Aspartic Acid

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrasnatQ61R

Plasmid

#206843

Purpose

To express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Glutamine 61 to Arginine

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrascomG12D

Plasmid

#206844

Purpose

To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Codons altered to most optimum based on mouse cod…

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαi1-91-NLuc

Plasmid

#207148

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAI1

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position L(91)K

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαi2-91-NLuc

Plasmid

#207149

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAI2

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position L(91)Q

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαi3-91-NLuc

Plasmid

#207150

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAI3

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position L(91)K

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)GαoA-91-NLuc

Plasmid

#207151

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAO

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position L(91)G

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)GαoB-91-NLuc

Plasmid

#207152

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAO

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position L(91)G

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-StrepHA-SUMO2(Q87R)

Plasmid

#66868

Purpose

Mammalian expression of SUMO2(Q87R) with a strep and an HA tag

Depositor

Ying Liu

Article

Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

Insert

SUMO2 (SUMO2 Human)

Use

Tags

HA and Strep

Expression

Mammalian

Mutation

Q87R

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-kappa-HA-dL5-BKa

Plasmid

#73212

Purpose

Expresses HA-dL5(E52D)-BKa (KCNMA1, mSlo alpha subunit) in mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Pratt et al Bioconjug Chem. 2015 Sep 16;26(9):1963-7

Insert

BKalpha (Kcnma1 Mouse)

Use

Tags

dL5**

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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mOC-STAMP_pcDNA3.1 D/V5-His-TOPO

Plasmid

#73448

Purpose

Express mouse OC-STAMP in mammalian cells

Depositor

Paul Odgren

Article

Yang et al J Cell Physiol. 2008 May;215(2):497-505.

Insert

Ocstamp (Ocstamp Mouse)

Use

Tags

V5-6xHis

Expression

Mammalian

Mutation

N162D

Promoter

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 Bach1 K141A/K142A

Plasmid

#73642

Purpose

Mammalian expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)

Depositor

Sharon Cantor

Insert

Bach1 (BACH1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

K141A/K142A ( mlh1 binding mutant)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only