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Plasmid#199757PurposeElongation reporter construct to quantify elongation of synYFP[CTT]DepositorInsertleuYFP[CTT]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-leuYFP[CTC]-nLuc
Plasmid#199758PurposeElongation reporter construct to quantify elongation of synYFP[CTC]DepositorInsertleuYFP[CTC]
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
50_p5E_UBI_loxTagBFP
Plasmid#172518Purposep5E tol2kit compatible clone encoding a Ubiquitin promoter and a tagBFP sequence terminated by 3x polyA sequences and flanked by floxP sites for CRE-excisionDepositorInsertubiquitin promoter, TagBFP, loxP sites (inversion)
UseTagsExpressionMutationPromoterUbiquitinAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH059
Plasmid#186641PurposeaadA.co-TaadA-PrbcL-eYFP.co between homology arms for pilADepositorArticleInsertaadA.co-TaadA-PrbcL-eYFP.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH139
Plasmid#186642PurposeaadA.co-TaadA-PJ23119-eYFP.co between homology arms for pilADepositorArticleInsertaadA.co-TaadA-PJ23119-eYFP.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH140
Plasmid#186643PurposeaadA.co-TaadA-PJ23111-eYFP.co between homology arms for pilADepositorArticleInsertaadA.co-TaadA-PJ23111-eYFP.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH141
Plasmid#186644PurposeaadA.co-TaadA-PJ23100-eYFP.co between homology arms for pilADepositorArticleInsertaadA.co-TaadA-PJ23100-eYFP.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH142
Plasmid#186645PurposeaadA.co-TaadA-PJ23107-eYFP.co between homology arms for pilADepositorArticleInsertaadA.co-TaadA-PJ23107-eYFP.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH143
Plasmid#186646PurposeaadA.co-TaadA-PJ23117-eYFP.co between homology arms for pilADepositorArticleInsertaadA.co-TaadA-PJ23117-eYFP.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCD86-mEYFP(Q69K)
Plasmid#190746PurposeExpression of mEYFP(Q69K)-tagged CD86 in mammalian cells.DepositorInsertCD86 (CD86 Human)
UseTagsmEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NODALvar
Plasmid#115640PurposeFor targeted integration and inducible expression of a human NODAL splice variant using doxycyclineDepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-matDYK
Plasmid#115258PurposeFor mammalian expression of the human NODAL open reading frame with an internal DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-matDYK
Plasmid#115260PurposeFor mammalian expression of a human NODAL splice varaint open reading frame with an internal DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-matNODALvar-HIS
Plasmid#115265PurposeFor bacterial recombinant protein production of the mature peptide of a human NODAL splice variantDepositorInsertNODAL variant mature peptide (NODAL Human)
UseTags6HisExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-matMYC
Plasmid#115249PurposeFor mammalian expression of a human NODAL splice variant open reading frame with an internal MYC tagDepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-T7TS
Plasmid#115250PurposeFor in vitro transcription of human NODAL open reading frame RNADepositorInsertNODAL (NODAL Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-T7TS
Plasmid#115251PurposeFor in vitro transcription of a human NODAL splice variant open reading frame RNADepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
His8OphMA-21mer.
Plasmid#189670PurposeHis8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.DepositorInsertHis8OphMATEV18mer
UseTagsHis8 taggedExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
His8OphMADeltaC6-18mer.
Plasmid#189671PurposeHis8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptidesDepositorInsertHis8OphMA∆C6-TEV18mer
UseTagsHis8 taggedExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTbp-Firefly
Plasmid#177835PurposeLuciferase reporter for mouse TbpDepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterTbpAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only