-
Plasmid#113200Purposeshuttle vector, C-terminal mScarlet taggingDepositorTypeEmpty backboneUseDictyostelium discoideum shuttle vector, c-termin…TagsmScarletExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only
-
pPI418
Plasmid#113199Purposeshuttle vector, N-terminal mScarlet taggingDepositorTypeEmpty backboneUseDictyostelium discoideum shuttle vector, n-termin…TagsmScarletExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_66.11
Plasmid#194213PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_69.11
Plasmid#194214PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL2_175
Plasmid#199107PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertDummy ORI plus NGS barcode 23, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_172
Plasmid#199104PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpSG5 ORI plus NGS barcode 20, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
DT039A
Plasmid#159400PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoterDepositorInsertpr.J23117-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterJ23117 (weak) promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT038A
Plasmid#159399PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23106 (medium) promoterDepositorInsertpr.J23106-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterJ23106 (medium) promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT040A
Plasmid#159401PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoterDepositorInsertpr.J23117/J23106-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterhybrid J23117-J23106 promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Akaluc-N1
Plasmid#120371PurposeAkaluc luciferase mammalian expression in -N1 backboneDepositorInsertAkaluc (human optimized)
UseTagsExpressionMammalianMutationHuman codon optimizedPromoterCMVAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ151-HDR
Plasmid#113631PurposeContains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.DepositorInsertsLeft homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm
UseCre/Lox; Homology recombination selection vectorTagsExpressionBacterial and MammalianMutationPromoterEF1a promoter, EF1a promoter (T2A cleaving sequen…Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
DT041A
Plasmid#159402PurposeConstitutive murine TrkB expression in mammalian host, controlled under cytomegalovirus (CMV) promoter and enhancerDepositorInsertpLenti-CMV-TrkB (Ntrk2, Mouse) (Ntrk2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-KASH2
Plasmid#187017PurposeInducible disruption of LINC ComplexDepositorInsertKASH2
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30a-FluB-NP
Plasmid#165093Purposeprotein expression in E. coliDepositorInsertInfluenza B virus(B/Georgia/05/2019) nucleocapsid protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-KASH2ext
Plasmid#187018PurposeControl for inducible disruption of LINC ComplexDepositorInsertKASH2ext
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
DT044A
Plasmid#159405PurposeInducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)DepositorInsertpr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
UseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterT7/LacO promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pSLHDR02_EGFP_tCD8
Plasmid#124061PurposeConstruction of 1st donor for scarless genome editingDepositorInsertsEGFP
tCD8
UseMouse Targeting; Human targetingTagsExpressionMutationPromoterHuman UbC promoter modifiedAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
KG#121
Plasmid#110879PurposeExpresses the C. elegans unc-31 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationMissing first 408bpPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrex-n-eGFP
Plasmid#62544PurposeExpression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genomeDepositorInserteGFP
Use(1)trypanosoma cruzi expression of egfp (2) genom…TagsNoneExpressionMutationNonePromoterRibo-HX1Available sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only