Showing: 1121 - 1140 of 1186 results
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Plasmid#53259PurposeSNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
UseTags4aa-linker and mCitrineExpressionMammalianMutationD179K and A199RPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Twitch-2B pcDNA3
Plasmid#49531PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-2B
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Twitch-3 pcDNA3
Plasmid#49532PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-3
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC
Plasmid#67687PurposeUAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassetteDepositorInsertdusp6 (dusp6 Zebrafish)
UseTags6xmyc-NLS-tdTomato-2AExpressionMutationS159A, S196APromoterAvailabilityAcademic Institutions and Nonprofits only -
pCerulean-VSVG
Plasmid#11913DepositorInsertVSVG
UseTagsExpressionMammalianMutationF64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…PromoterAvailabilityAcademic Institutions and Nonprofits only -
Cerulean-GalT
Plasmid#11930DepositorInsertGalT (GALT Human)
UseTagsExpressionMammalianMutationAmino acids 1-60 of the human galactosyltransfera…PromoterAvailabilityAcademic Institutions and Nonprofits only -
Lyn FAK biosensor
Plasmid#78299PurposeFRET biosensor. Visualization of FAK activity at lipid rafts in live cellsDepositorInsertLyn Fak biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
KRas FAK biosensor
Plasmid#78301PurposeFRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cellsDepositorInsertKRas FAK biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyTagsExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailabilityAcademic Institutions and Nonprofits only -
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pCS-CG-Lock-Cer-VWF
Plasmid#124828PurposeExpresses multimeric human VWF-ECFP fusion protein with A2-Lock domain The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.DepositorInsertVWF with cyan fluorescent protein fusion between D'D3 and A1 domains and Lock A2 domain (VWF Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pQE60-mPU.1-ETS
Plasmid#85733PurposeExpresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4DepositorInsertETS domain of transcription factor PU.1 (Spi1 Mouse)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
cfmTurquoise-N
Plasmid#139284PurposeCys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.DepositorInsertcfmTurquoise
UseTagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-Pu.1
Plasmid#139839Purposedoxycycline-inducible expression of mouse Pu.1 in mammalian cellsDepositorInsertPu.1 (Spi1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise-parkin
Plasmid#110945PurposeExpresses the ubiquitin ligase parkin fused to mTurquoiseDepositorInsertE3 ubiquitin ligase parkin (PRKN Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-Venus-Actb
Plasmid#169788PurposeAn actin FRET sensor. Must be expressed with ECFP-actin for FRET.DepositorInsertActb (Actb Mouse)
UseTagsVenusExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pSLQ10844
Plasmid#183956PurposeCAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAGAvailabilityAcademic Institutions and Nonprofits only
Showing: 1121 - 1140 of 1186 results