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Plasmid#63209PurposepCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only
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pOPS0378
Plasmid#133229PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(276-598)
Plasmid#135437PurposeExpresses SFPQ 276-598 with TEV-cleavable hexahistidine tagDepositorInsertSplicing Factor Proline And Glutamine Rich (SFPQ Human)
UseTagshexahistidineExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSCT (JBEI-11557)
Plasmid#87937PurposeCo-expression in S.cerevisiae of 4CL5 and AtSCTDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH7WG2B-OsMIR390-B/c
Plasmid#61465PurposePlant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a cā¦PromoterOsUBIAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K5
Plasmid#63210PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV10.K4
Plasmid#63212PurposepCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV11K
Plasmid#63184PurposepRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Enhancer-ZC3H12D_PromoterWT
Plasmid#153066PurposeZC3H12D promoter region cloned upstream of luciferase reporter geneDepositorInsertZC3H12D promoter region (ZC3H12D Human)
UseLuciferaseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-OsMIR390-B/c
Plasmid#61467PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a cā¦Promoter2x35SAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pfastbacHTb/mClock26-384
Plasmid#47336PurposemClock fragment cloned into pfastbacHTb vector with Hexa His tagDepositorInsertClock (Clock Mouse)
UseTagsHis and TevExpressionMutationaa 26-384PromoterPHAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMDC123SB-OsMIR390-B/c
Plasmid#61466PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a cā¦Promoter2x35SAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-OsMIR390-B/c
Plasmid#61468PurposeGateway-compatible entry vector for direct cloning of artificial microRNAs into rice MIR390 precursor.DepositorInsertOsMIR390-B/c
UseGateway entry vectorTagsExpressionMutationO. sativa MIR390 precursor sequence including a cā¦PromoterAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(214-598)
Plasmid#135440PurposeExpresses SFPQ 214-598 with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
UseTagsHexahistidineExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(276-535)
Plasmid#135436PurposeExpresses SFPQ 276-535 with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
UseTagsHexahistidineExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASp-FMW-attB-AthHen1
Plasmid#104957PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 driversDepositorInsertHen1 (HEN1 Mustard Weed)
UseTagsFLAG MycExpressionInsectMutationPromoterpTrasposase promoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV10/3xflag-Clock L57E
Plasmid#47337PurposeMutation used for co-IPsDepositorInsertClock (Clock Mouse)
UseTags3x FlagExpressionMammalianMutationL57EPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV10/3xflag-Clock L74E
Plasmid#47338PurposeMutation used for co-IPsDepositorInsertClock (Clock Mouse)
UseTags3x FlagExpressionMammalianMutationL74EPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3
Plasmid#63199PurposeBioBrick pSB1C3 backbone (Cm), HIS3, RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-WRAP53beta-WD40domain
Plasmid#64678PurposeExpresses the WD40 domain of WRAP53betaDepositorInsertWd40-encoding RNA antisense to P53 (WRAP53 Human)
UseTagsGFPExpressionMammalianMutationcontaining only WD40 domain of WRAP53betaPromoterCMVAvailable sinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV10/3xflag-Clock V315R
Plasmid#47342PurposeMutation used for co-IPsDepositorInsertClock (Clock Mouse)
UseTags3x FlagExpressionMammalianMutationV315RPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV10/3xflag-Clock W284A
Plasmid#47341PurposeMutation used for co-IPsDepositorInsertClock (Clock Mouse)
UseTags3x FlagExpressionMammalianMutationW284APromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Bov.hsc70.RSET.a1-554.wt(NarI)
Plasmid#12533DepositorInsertBovine 70 kDa heat shock cognate protein fragment amino acids 1-554
UseTagsExpressionBacterialMutationdeleted carboxy-terminal residues 555-650 of boviā¦PromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDF11-SFPQ(369-598)
Plasmid#135438PurposeExpresses SFPQ 369-598 with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
UseTagsHexahistidineExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOGG043
Plasmid#133123PurposeLevel 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.DepositorInsertPsNifH Synthetic consensus nifH promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV113.loxP
Plasmid#63186PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV115.loxP
Plasmid#63187PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV116.loxP
Plasmid#63188PurposepRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only