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Plasmid#127369PurposeBinary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plantsDepositorInsertminiTurbo (BirA mutant)
UseTagsGS linker, NES, V5, and YFPExpressionPlantMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEE065
Plasmid#176822PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert704
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE062
Plasmid#176819PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert701
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tn7 integration plasmid
Plasmid#154134PurposeSuicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.DepositorInsertsleft and right end of Tn7 arm
Spectinomycin resistant gene
UseTagsExpressionBacterialMutationPromoterSpectinomycin resistance gene promoter and noAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TLR targeting vector
Plasmid#64215PurposeTargeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) constructDepositorInserttraffic light reporter (TLR) (AAVS1 Human)
UseHuman targetingTagsExpressionMutationsee publication for detailsPromoterCAGAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas9(D10A)
Plasmid#74495PurposeNicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ADepositorInsertCas9 D10A
UseSynthetic BiologyTagsExpressionBacterialMutationD10APromoterpTetoAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMis1_1B
Plasmid#192079PurposeA pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent inductionDepositorInsertsKanR
rhaR
rhaS
pBBR1 rep
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
cyt-GafD-mTurboID-V5
Plasmid#184640PurposeExpresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome taggingDepositorInsertcyt-GlycoID-V5
UseTagsNuclear export sequence and V5 tagExpressionMammalianMutationGafD -> expressed aa22-178 ; BirA* -> expre…PromoterCMVAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE077
Plasmid#176834PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert716
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 P-cad
Plasmid#47502PurposeExpresses P-cadherin in mammalian cellsDepositorInsertcadherin 3, type 1, P-cadherin (placental) (CDH3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
RANLS
Plasmid#50843PurposeExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.DepositorInsertddRFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
FSW-HRP-V5-LRRTM2
Plasmid#82537PurposeLentiviral vector for expressing HRP-LRRTM2 in neuronsDepositorInsertLRRTM2 (LRRTM2 Human)
UseLentiviralTagsHRP and V5ExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-mPLD4
Plasmid#201185PurposeProtein expression plasmid for recombinant His-Myc tag mouse PLD4DepositorUseTagsHis-Myc, Factor Xa cleavableExpressionMammalianMutationcytosolic and transmembrane domain truncated; add…PromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-erExRai-AKAR2
Plasmid#161756PurposeExRai-AKAR2 targeted to the cytosolic face of the ER membrane.DepositorInsertCYP450-ExRai-AKAR2
UseTagsN-terminal 27 amino acids from CYP450ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Vimentin - APEX2 in pECFP
Plasmid#66170PurposeAPEX2 fused to the C-terminus human vimentinDepositorInserthuman Vimentin - APEX2 (VIM Synthetic, Human)
UseTagsFlag epitope tag (DYKDDDDK)ExpressionMammalianMutationAPEX2 contains 4 mutations relative to wt soybean…PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
BNLS
Plasmid#50836PurposeExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).DepositorInsertddGFP B
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
FSW-HRP-V5-LRRTM1
Plasmid#82536PurposeLentiviral vector for expressing HRP-LRRTM1 in neuronsDepositorInsertLRRTM1 (LRRTM1 Human)
UseLentiviralTagsHRP and V5ExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
GANLS
Plasmid#50852PurposeExpresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.DepositorInsertddGFP A
UseTagstriplicated NLS sequence DPKKKRKVExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Fv-hCaspase 8 (C/A)-2A-GFP
Plasmid#82713Purposeexpress human caspase 8 in mammalian cellsDepositorInsertFv-Caspase 8 (C/A)-2A-GFP (CASP8 Human)
UseRetroviralTagsFKBP dimerization domainExpressionMammalianMutationdeleted amino acids 1-215 of caspase 8PromoterAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only