Showing: 11321 - 11340 of 11517 results
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Plasmid#215000PurposeEndogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.DepositorInsertmsTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
131-2a_LC
Plasmid#215331PurposeExpression monoclonal antibody 131-2a LCDepositorInsert131-2a_LC
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Msi1_2xRRM_pGEX
Plasmid#215704PurposeRecombinant expression of mouse Msi1 with dual-affinity tagDepositorInsertMusashi-1 (Msi1 Mouse)
UseTagsGST and SBPExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pColdI-GST-mGTSF2
Plasmid#215714PurposeBacterial expression of GST-tagged GTSF2DepositorInsertGTSF2 (Gtsf2 Mouse)
UseTags6x His and GSTExpressionBacterialMutationPromoterCspAAvailabilityAcademic Institutions and Nonprofits only -
2xHP1bCD-IFP2.0C
Plasmid#215750PurposeExpresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensorsDepositorInsert2xHP1bCD-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
2xHP1bCD_W42A-IFP2.0C
Plasmid#215751PurposeExpresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensorsDepositorInsert2xHP1bCD_W42A-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated
Plasmid#215769Purposeencodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locusDepositorInsertsUseTagsExpressionBacterialMutationCas12a PAM mutatedPromoterAvailabilityAcademic Institutions and Nonprofits only -
2C-EGFP-t2a-mCD4
Plasmid#216213PurposeExpresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoterDepositorInsertCD4 (Cd4 Mouse)
UseTagsGFP-T2AExpressionMammalianMutationCD4 transmembrane domain onlyPromoterMERVLAvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
Plasmid#216378PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTagsHA signal sequence-FLAGExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
mFzd4CRDL(42-203)-6xHis_pAcGP67a
Plasmid#216379PurposeBaculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cellsDepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags6xHis and GP64 signal sequenceExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393
Plasmid#216380PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTags3C-splitGFP1to10opt-ETVV and HA signal sequence-F…ExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet
Plasmid#216386PurposeBacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)DepositorInsertDishevelled-2 (Dvl2 Mouse)
UseTags6xHis-MBP-TEVExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet
Plasmid#216387PurposeBacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)DepositorInsertDishevelled-2 (Dvl2 Mouse)
UseTags6xHis-MBP-TEVExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag
Plasmid#216695PurposeSynchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)DepositorInsertsUseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
CIRTS-FTO-control
Plasmid#216859PurposeCIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-FTO-Calm3-U6-control gRNA
UseLentiviralTagsmCherry2ExpressionMammalianMutationPromoterSyn1, U6AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-FTO-Malat1
Plasmid#216860PurposeCIRTS RNA targeting system for targeted removal of m6A on Malat1 at the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-FTO-Calm3-U6-Malat1 gRNA
UseLentiviralTagsExpressionMammalianMutationPromoterSyn1, U6AvailabilityAcademic Institutions and Nonprofits only -
Lenti-iStat3-BFP
Plasmid#216870PurposeLenti-iStat3-BFP plasmid contains the TRE (doxycycline inducible) promoter followed by the Stat3 mouse cDNA, a T2A and a Blue Fluorescent Protein (BFP) sequence.DepositorInsertStat3 (Stat3 Mouse)
UseLentiviralTagsT2A-TagBFPExpressionMammalianMutationPromoterTetracycline-Response-Element (TRE)AvailabilityAcademic Institutions and Nonprofits only -
pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)
Plasmid#216871PurposeThis plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23DepositorInsertCas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
UseCRISPRTagsExpressionMutationmdx mutation in mouse dystrophinPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAGGS 3xFLAG Mbnl2
Plasmid#217032PurposeOverexpression of Mbnl2 in mammalian cellsDepositorInsertMbnl2 (Mbnl2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Lenti-Nova1-GFP
Plasmid#217033PurposeOverexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neuronsDepositorInsertNova1 (Nova1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 11321 - 11340 of 11517 results