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Showing: 11321 - 11340 of 11908 results
  1. pENTR_L1-Turbo-NES-YFP-STOP-L2

    Plasmid
    #127358
    Purpose
    gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice
    Depositor
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pLGB36

    Plasmid
    #135621
    Purpose
    Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection
    Depositor
    Insert
    bfe3
    Use
    Bacteroides suicide vector with inducible counter…
    Tags
    signal sequence for periplasmic targeting
    Expression
    Mutation
    Promoter
    Bacteroides promoter regualted by TetR transcript…
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. RS35075_pMQ30_KO_Kan

    Plasmid
    #185390
    Purpose
    Non-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11
    Depositor
    Inserts
    RR42_RS35075 upstream flanking homology region
    RR42_RS35075 downstream flanking homology region
    nptII kanamycin resistance marker
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid
    #83239
    Purpose
    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.
    Depositor
    Insert
    roGFP2-Tsa2dCpdCr
    Use
    Tags
    roGFP2 fusion with Tsa2dCpdCr
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. pJG367

    Plasmid
    #91185
    Purpose
    BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)
    Depositor
    Insert
    nAtCas9_H840A+gNt_R2+gNt_F2+donor
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pJG382

    Plasmid
    #91189
    Purpose
    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)
    Depositor
    Insert
    nAtCas9_D10A+gNt_R2+gNt_F2+donor
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pS44i8GM

    Plasmid
    #207527
    Purpose
    Conditionally-replicating in Pseudomonas vector, medium-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR
    Depositor
    Insert
    oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 11321 - 11340 of 11908 results