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152,174 results

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LD127

Plasmid

#69175

Purpose

Integration of GFP tag at Atg27 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg27-EGFP

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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UAS-FRT-GFP-FRT-u(M)Cas9

Plasmid

#127388

Purpose

Cas9 inducible by removal of a GFP flip-out cassette

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

FRT-GFP-FRT-uORF(M)Cas9

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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mRFP1-N1

Plasmid

#54635

Purpose

Localization: N1 Cloning Vector, Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Use

Tags

mRFP1

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+-HypocratesCS

Plasmid

#161960

Purpose

Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

Depositor

Dmitry Bilan

Article

Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

Insert

Hypocrates

Use

Tags

Expression

Mammalian

Mutation

changed Cysteine 355 to Serine

Promoter

CMV, SP6

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Venus-Fluc (puro)

Plasmid

#140328

Purpose

Lentivirus for expression of Venus-Fluc (firefly luciferase) fusion protein

Depositor

Roland Friedel

Article

Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

Insert

Venus-Firefly Luciferase (luc2)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human PGK promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-FLPo-3xP3-RFP

Plasmid

#127561

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

FLPo

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-mDLX-TVA-2A-N2cG

Plasmid

#172364

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neurons

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-N2cG

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mDLX

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-RhoA

Plasmid

#12202

Depositor

Jonathan Chernoff

Article

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

RhoA (RHOA Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-mTmod3-WT

Plasmid

#70753

Purpose

mouse Tropomodulin3, WT for bacterial expression

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 (Tmod3 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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EC71022

Plasmid

#159608

Purpose

Level 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

loxP-mCherry-HDEL-t35S-loxP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Promoter

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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