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Plasmid#134660PurposeCRISPR delivery and repair template delivery vectorDepositorInsertCRISPR guide RNA array
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK317
Plasmid#59571PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZR060_CROPseq-sgCtrl-CRISPRi
Plasmid#180269PurposeLentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRiDepositorInsertNo-Target Control sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-IRES-eGFP
Plasmid#80343PurposeExpresses smURFP and eGFP in mammalian cells.DepositorInsertssmURFP
eGFP
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TLR6
Plasmid#118997PurposeTargeting vector for human AAVS1 with TLR6 reporter insertDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CaMKIIP-mEGFP-P2A-paAIP2/pAAV
Plasmid#91718PurposeAAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKIIDepositorInsertmEGFP-P2A-paAIP2
UseAAVTagsExpressionMutationPromoterCaMKII 1.3Available sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA(123)
Plasmid#102613PurposeExpression of human RPA in E. coliDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pANAP
Plasmid#48696Purposeallows co-translational incorporation of a fluorescent AA directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
UseTagsExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy55
Plasmid#122007PurposeMammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis10-SumoStar (Life Sensors) and Multi2: dependi…ExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_mNG2(11)_Clathrin light chain
Plasmid#82608PurposeExpresses mNG2(11) tagged clathrin light chain in mammalian cellsDepositorInsertmNG2(11)_Clathrin light chain
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNatB (pACYCduet-naa20-naa25)
Plasmid#53613PurposeAllows expression of the fission yeast NatB complex - chloramphenicol markerDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKIR1.1
Plasmid#85758PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporterDepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAGExpressionPlantMutationPromoterAtRPS5AAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
SupD v2
Plasmid#198598PurposePlasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.DepositorInsertSupD
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFD4-U6:1_U6:3tandemgRNAs
Plasmid#49411Purposeexpression of two gRNAs from Drosophila U6:1 and U6:3DepositorInsertsdU6-1:gRNA
dU6-3:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6-1 and dU6-3Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_gRNA
Plasmid#80457PurposegRNA expression vector with mCherry transfection controlDepositorInsertssp Cas9 gRNA
mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterU6 and chicken β-actin promoterAvailable sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_ENTR_12GLI-FLuc_R4-R3
Plasmid#113712PurposeSingle pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.DepositorInsert12GLI-FLuc
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
Plasmid#74317PurposeA fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafishDepositorInsertssynaptophysin
DsRedExpress
UseTagsEGFPExpressionMutationPromoter5UASAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Halo-GPI-RUSH
Plasmid#166903PurposeRUSH cargo: Str-KDEL_IRES_SBP-Halo-GPIDepositorInsertHaloTag
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIB VP64 R1
Plasmid#161493PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting R1 in the RIB1 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
murine TCR OTII-2A.pMIG II
Plasmid#52112Purposeretroviral expression of both TCRalpha and TCRbeta using a single vectorDepositorUseRetroviralTagsP2A (from porcine teschovirus-1)ExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only