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pHS1182

Plasmid

#205964

Purpose

NZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH crRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgGFP-1

Plasmid

#74960

Purpose

Cas9 + sgGFP-1 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

GFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

Plasmid

#98975

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 4 and Cas9(D10A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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lentiviral shRNA GEF-H1

Plasmid

#21477

Depositor

Richard Huganir

Article

Kang et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

Insert

rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pLKO mouse ULK2 shRNA 93

Plasmid

#27635

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse ULK2 shRNA 93

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
pLVTHM-shN

Plasmid

#83046

Purpose

Control shRNA pLVTHM-shN

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA Neg Control

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE-H1

Available since

Oct. 24, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pSMP-EZH1_1

Plasmid

#36359

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH1 (EZH1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
shPBRM1#32-Tet-pLKO-puro

Plasmid

#107399

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSico-shRaptor

Plasmid

#81086

Purpose

conditional expression of an shRNA targeting murine Raptor

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

Raptor shRNA (Rptor Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only

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RT3GEN

Plasmid

#111165

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

T3G

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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sgLenti-orthogonal

Plasmid

#105997

Purpose

Dual Streptococcus pyogenes and Staphylococcus aureus sgRNA expression vector

Depositor

Mike McManus

Article

Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

Type

Empty backbone

Use

CRISPR and Lentiviral; S.pyogenes sgrna expressio…

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pHS1528

Plasmid

#205982

Purpose

U6-MWEE01000013 Cas5-HNH DR-BpiI-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH DR-BpiI-DR

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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shPBRM1#131-Tet-pLKO-puro

Plasmid

#107400

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSMP-G9A_1

Plasmid

#36395

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pPV-H1-ccdB-mEF1α-RiH

Plasmid

#100598

Purpose

piggyBac vector for sgRNA cloning

Depositor

Akitsu Hotta

Article

Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

Type

Empty backbone

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Human H1 PolIII promoter, human FTH1 promoter, mo…

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 v2 neo

Plasmid

#72570

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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sh-RNA hAtg13

Plasmid

#31971

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pXPR_003 Intergenic control guide 2

Plasmid

#193585

Purpose

sgRNA control; induces CAS9 cutting in an intergenic region

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgRNA control

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgNFkB2 guide 2

Plasmid

#193594

Purpose

NFkB2 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgNFkB2 guide 2 (NFKB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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tdTomato/pTREX-b

Plasmid

#68709

Purpose

Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Insert

tdTomato

Use

Expression of tdtomato red fluorescence protein i…

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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