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Plasmid#175759PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-CALCOCO2/NDP52DepositorInsertNDP52/CALCOCO2 (CALCOCO2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAWH
Plasmid#133894PurposeExpression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22HDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GDI2
Plasmid#67093PurposeStandard for cell-free expression benchmarking.DepositorInsertGDI2 (GDI2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-Y419F
Plasmid#140317PurposeExpresses Flag-tagged human Src-Y419F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationY419FPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pUB-cSypHer
Plasmid#84733PurposeExpresses SypHer in plant cells-biosensor for pHDepositorInsertSypHer
UseTagsExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL1B
Plasmid#67066PurposeStandard for cell-free expression benchmarking.DepositorInsertIL1B (IL1B Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_INSR
Plasmid#187788PurposeMAC-tagged gene expression of human INSRDepositorInsertINSR (INSR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G06_ZIKV NS5
Plasmid#145358PurposeCell-free expression vector for Zika virus NS5 protein with C-term mCherryDepositorInsertZIKV NS5
UseTagsmCherry and 8xHisExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-Sox17
Plasmid#60669PurposePiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoterDepositorInsertSox17 (Sox17 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-QUAS5x
Plasmid#155113Purpose5' entry vector containing QUAS5x promoter element.DepositorInsertQUAS5x
UseGateway 5' entry vectorTagsExpressionMutationPromoterQUAS5x-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
p6898 PHAGE-P CMVT N-HA-hE6AP I C820A
Plasmid#37602DepositorInsertE6AP I C820A (UBE3A Human)
UseTagsHAExpressionMammalianMutationC820APromoterCMV tet regulatableAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAC1821_pCR8-SMN2-DN-RG6-SA
Plasmid#118655PurposeTransient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway DonorDepositorInsertSMN2-DN-RG6-SA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CIRBP
Plasmid#67055PurposeStandard for cell-free expression benchmarking.DepositorInsertCIRBP (CIRBP Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-cmlc2-Trpa1b-2A-EGFP
Plasmid#106405PurposeFor expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.DepositorInsertsUseGatewayTags2A-EGFPExpressionMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ macroH2A1.2-GFP-HA
Plasmid#30515DepositorInsertmacroH2A1.2-C (Macroh2a1 Mouse)
UseXenopus/zebrafish expression.TagseGFP and triple HAExpressionMammalianMutationNO STOPPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
Tol2-ngn1-Trpa1b-2A-mCherry
Plasmid#106425PurposeFor expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.DepositorInsertsUseGatewayTags2A-mCherryExpressionMutationPromoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-K298M
Plasmid#140315PurposeExpresses Flag-tagged human Src-K298M in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationK298MPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Gg
Plasmid#61653PurposeGateway vector for generating green fluorescent protein fusions in fungiDepositorInsertenhanced green fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGATOR-DTA
Plasmid#64103PurposeTet inducible, Gateway adapted ROSA26 targeting Vector with DTADepositorInsertrTTA (Gt(ROSA)26Sor Synthetic)
UseMouse Targeting; Tet inducibleTagsExpressionMammalianMutationPromoterROSA26 endogenousAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only