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PGM2L1 gRNA (BRDN0001149301)

Plasmid

#77145

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGM2L1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGM2L1 (PGM2L1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF1-S400F_RASA2-EF1-Puro

Plasmid

#114098

Purpose

Express mutant RASA2 in mamalian cells

Depositor

Yardena Samuels

Article

Arafeh et al Nat Genet. 2015 Dec;47(12):1408-10. doi:

Insert

RASA2 (RASA2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S400F

Promoter

CMV

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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sgRNA4_NANOG

Plasmid

#64156

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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TXK gRNA (BRDN0001146212)

Plasmid

#76360

Purpose

3rd generation lentiviral gRNA plasmid targeting human TXK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TXK (TXK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TXK gRNA (BRDN0001145866)

Plasmid

#76359

Purpose

3rd generation lentiviral gRNA plasmid targeting human TXK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TXK (TXK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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TXK gRNA (BRDN0001145857)

Plasmid

#76358

Purpose

3rd generation lentiviral gRNA plasmid targeting human TXK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TXK (TXK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF12_P2A_Hygro_Barcode

Plasmid

#120498

Purpose

Barcoded lentiviral vector to express KLF12 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF12 (KLF12 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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CSF1R gRNA (BRDN0001146892)

Plasmid

#77039

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSF1R

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSF1R (CSF1R Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-A598_T599insP

Plasmid

#116131

Purpose

Lentiviral expression of BRAF A598_T599insP

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

Expression

Mutation

A598_T599insP

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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IRAK1 gRNA (BRDN0001147615)

Plasmid

#76419

Purpose

3rd generation lentiviral gRNA plasmid targeting human IRAK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IRAK1 (IRAK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IRAK3 gRNA (BRDN0001146930)

Plasmid

#77714

Purpose

3rd generation lentiviral gRNA plasmid targeting human IRAK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IRAK3 (IRAK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IRAK3 gRNA (BRDN0001146977)

Plasmid

#77715

Purpose

3rd generation lentiviral gRNA plasmid targeting human IRAK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IRAK3 (IRAK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgFTCD_1

Plasmid

#102312

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_1 (FTCD Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgFTCD_2

Plasmid

#102313

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_2 (FTCD Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti4/V5-DEST-P85(DKRMNS560del)

Plasmid

#40225

Depositor

Lynda Chin

Insert

P85(DKRMNS560del) (PIK3R1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 560-565

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
PLAU gRNA (BRDN0001147652)

Plasmid

#77102

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLAU

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PLAU (PLAU Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLENTI-PGK-HYGRO-GATA6mut-HA

Plasmid

#65476

Purpose

Lentiviral expression of GATA6 Znc-finger mutant

Depositor

Eduard Batlle

Article

Whissell et al Nat Cell Biol. 2014 Jul;16(7):695-707. d

Insert

GATA6mut (GATA6 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

lacking the eight most highly conserved bases of …

Promoter

PGK

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCR1110

Plasmid

#111096

Purpose

Expresses BLM_+_91260645.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

BLM gRNA (BLM Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hSesn2 R419A

Plasmid

#111792

Purpose

Mammalian expression or 3rd generation lentivirus generation of hSesn2

Depositor

Jun Hee Lee

Article

Kim et al Nat Commun. 2015 Nov 27;6:10025. doi: 10

Insert

hSesn2 (SESN2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R419A

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev

Plasmid

#101340

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, tat, rev

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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