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pCIG1_UL136_23kDa_Myc

Plasmid

#74949

Purpose

Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Insert

UL136 (UL136 Human Cytomegalovirus strain TB40/E)

Use

Lentiviral

Tags

HA Tag (YPYDVPDYA)

Expression

Mammalian

Mutation

delta 1-99 M128A

Promoter

CMV, Lentiviral LTR

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3- myc NES-mCE(K294A) NLS

Plasmid

#82467

Purpose

Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NLS deleted from mouse Capping Enzyme (Rngtt Mouse)

Use

Tags

Expression

Mammalian

Mutation

deletion of KRKY from 573-657

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgAMDHD1

Plasmid

#102316

Purpose

genetic depletion of AMDHD1

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgAMDHD1 (AMDHD1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-dCys-T46C

Plasmid

#110543

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

T46 mutated to C46, C85 mutated to A85, C152 muta…

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-dCys-L54C

Plasmid

#110542

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

L54 mutated to C54, C85 mutated to A85, C152 muta…

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-D27S/Y100F

Plasmid

#110827

Purpose

Bacterial expression of E.coli DHFR D27S/Y100F mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

D27 mutated to S27, Y100 mutated to F100

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm- 227

Plasmid

#90498

Purpose

pcDNA3 - CMVmin - VPR - PIF3, expresses VPR transactivator domain and PIF3, under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

VP64-PIF3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-113

Plasmid

#90495

Purpose

pcDNA3 -pCMV - MTAD - PIF6, expresses the Phytochrome interacting factor 6 (PIF6) and MTAD, under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

MTAD-PIF6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pWP1_mHAL

Plasmid

#102318

Purpose

expression of mouse cDNA of HAL refractory to the guide in plasmid 4

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Hal (Hal Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1alpha

Available since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_4

Plasmid

#73536

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 human A3C.S61P

Plasmid

#105048

Purpose

Overexpression of APOBEC3C S61P mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

serine 61 to proline

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.D99E

Plasmid

#105049

Purpose

Overexpression of APOBEC3C D99E mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Aspartate 99 to glutamate

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.DS-EP

Plasmid

#105050

Purpose

Overexpression of APOBEC3C DS-EP mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

S61P, D99E

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.C97S

Plasmid

#105051

Purpose

Overexpression of APOBEC3C C97S mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

cysteine 97 to serine

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 N181-211 (#595)

Plasmid

#99939

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delAA181-211insNAAIRS linker

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 N187-207 (#596)

Plasmid

#99940

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delAA187-207insNAAIRS linker

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 1-209 (#430)

Plasmid

#99926

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C-terminal deletion; encodes AA 1-209

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 96-110 (#189)

Plasmid

#99929

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Internal deletion of AA 96-110

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 111-135 (#190)

Plasmid

#99930

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Internal deletion of AA 111-135

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-REDD1 136-153 (#191)

Plasmid

#99931

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Internal deletion of AA 136-153

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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