Addgene

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pEGFP-N3/hArf5(WT)-EGFP

Plasmid

#79534

Purpose

Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf5 (ARF5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLV V5-TbID-LBR

Plasmid

#175100

Purpose

Lentiviral expression of V5-TbID-tagged mouse Lbr

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Lbr (Lbr Mouse)

Use

Lentiviral

Tags

V5-TbID

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC35F5

Plasmid

#132085

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35F5 (SLC35F5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Flag-M4-AR-S650D

Plasmid

#171246

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Ser650Asp (AR-S650D)

Promoter

CMV

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-S650A

Plasmid

#171245

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Ser650Ala (AR-S650A)

Promoter

CMV

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S81A

Plasmid

#171248

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)

Promoter

CMV

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S81D

Plasmid

#171249

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)

Promoter

CMV

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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EFNB2-tdTomato

Plasmid

#139967

Purpose

Donor vector to knockin tdTomado into EFNB2 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

Human EFNB2 knockin homology arms (EFNB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1_sh_ex13

Plasmid

#35165

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pGLS3-5xHRE-p53(K164R)

Plasmid

#72555

Purpose

Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K164R.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

p53 (TP53 Human)

Use

Tags

5XHRE

Expression

Mammalian

Mutation

K164R

Promoter

E1B minimal promoter

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC2A7

Plasmid

#132066

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A7 (SLC2A7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR1A-PIK3CA E545K

Plasmid

#180032

Purpose

Entry vector of PIK3CA E545K for gateway cloning.

Depositor

Xin Chen

Article

Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

Insert

PIK3CA E545K (PIK3CA Human)

Use

Entry vector

Tags

HA tag

Expression

Mammalian

Mutation

E545K

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC35A3

Plasmid

#132135

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A3 (SLC35A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDUAL-Pnmt1-Ost4-GFP-MTn

Plasmid

#154369

Purpose

Yeast expression of Ost4

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Ost4 (ost4 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-BHLHE40(S243A/E248A)

Plasmid

#110155

Purpose

Expression of human BHLHE40 (S243A/E248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

Insert

BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S243A/E248A

Promoter

CMV

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4D-CD4-FLAG

Plasmid

#51601

Purpose

expresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D/CD4-FLAG (Sema4d Human, Mouse)

Use

Tags

FLAG and myc

Expression

Mammalian

Mutation

Sema4D amino acids 660-861 were replaced with CD4…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223-GOLPH3

Plasmid

#21688

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

last nucleotide of stop codon removed for c-termi…

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pCR8/GW/TOPO-ELK1

Plasmid

#98616

Purpose

Gateway entry TOPO TA cloning vector for ELK1

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ELK1 (Elk1 Mouse)

Use

Topo ta cloning vector

Tags

Expression

Mutation

Q230R

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A52

Plasmid

#132031

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A52 (SLC25A52 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
tdEos-Actin-C-18

Plasmid

#57571

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Actin (ACTB Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits