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Plasmid#90124PurposeGateway Mid-entry clone containing promoter sequence specific for cldn5b driving expression of eGFPDepositorInsertcldn5bP1Egfp (cldn5b Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
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p5Etmem88aE1
Plasmid#90112Purpose5' Gateway entry clone containing endothelial specific enhancer sequence of tmem88aDepositorInserttmem88aE1 (tmem88a Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-OSF-TRIM5-21R(1-300)
Plasmid#79029PurposeGateway entry vector for cloning and propagation in DH5alphaDepositorInsertTRIM5-21R (1-300)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterT7Available sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-016
Plasmid#78704PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-015
Plasmid#78703PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-010
Plasmid#78697PurposeA strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-008
Plasmid#78695PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-007
Plasmid#78694PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB(KTF)-HA no-pA
Plasmid#82596Purpose3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationW2101FPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only