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Plasmid#45797PurposeFluorescent reporter for calcium signalingDepositorInsertTN-XXL
UseTagsECFP and YFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
CXXC1
Plasmid#32045PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCXXC1 (CXXC1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationCXXC domain (amino acids residues 161-222)PromoterAvailable sinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
TN-XXL pRSETB
Plasmid#45796PurposeFluorescent reporter for calcium signalingDepositorInsertTN-XXL
UseTags6xHIS, ECFP, and YFPExpressionBacterialMutationPromoterT7Available sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmiReport PU.1 3' UTR
Plasmid#26530DepositorInsertPU.1 3' UTR (Spi1 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSWD95
Plasmid#158617PurposeCckA FRET SensorDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationPromoterXyloseAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Twitch-1 pcDNA3
Plasmid#49812PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-1
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGWB445
Plasmid#74831PurposeGateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB444
Plasmid#74830PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
15x-QUAS-dTomato-T2A-TRPV1
Plasmid#140945PurposeQUAS reporter line that expresses cytosolic dTomato and the rat TRPV1 ion channel, separated by T2ADepositorInsert15x-QUAS-dTomato-T2A-TRPV1
UseTagsExpressionMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCit-4aa-SNAP25B
Plasmid#53235PurposeFluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
UseTags4aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Accessory plasmid 1
Plasmid#128569Purposefor mixing with SPECS library to produce lentivirusDepositorInsertECFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic FAK biosensor
Plasmid#78300PurposeFRET biosensor. Visualization of cytosolic FAK activity in live cellsDepositorInsertCytosolic FAK biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCer-4aa-VAMP2
Plasmid#53233PurposeFluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags4aa-linker and mCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3
Plasmid#61622PurposeFRET-based biosensor for monitoring cAMPDepositorInsertICUE3 (RAPGEF3 Human)
UseTagsECFP and cpVenus[L194]ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB544
Plasmid#74878PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-12aa-mCit
Plasmid#53237PurposeFluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.DepositorInsertVAMP2 (Vamp2 Rat)
UseTags12aa-linker and mCitrineExpressionMammalianMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
Twitch-4 pcDNA3
Plasmid#49533PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-4
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGWB543
Plasmid#74877PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV PIG Pu.1
Plasmid#66982PurposeRetroviral expression plasmid for mouse Pu.1 expression; containing bicistronic IRES-GFPDepositorInsertSpi1 (Spi1 Mouse)
UseRetroviralTagsExpressionMutationPromoterLTRAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Fyn-Fyn Biosensor
Plasmid#124655PurposeFRET biosensor. Fyn-tagged biosensor targeting to membraneDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only