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Plasmid#179000PurposeSp.Cas9 and gRNA yeast expression vector with ARS416 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)
Plasmid#178999PurposeSp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-F1_array_GG
Plasmid#178770PurposeGolden Gate vector to generate X. albilineans type I-F1 single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-F1 single arrays
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sth_I-E array_GFP dropout
Plasmid#178775PurposeGolden Gate GFP dropout vector to generate S. thermophilus type I-E single arraysDepositorInsertgfp dropout to generate S. thermophilus type I-E single arrays
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEh_gRNA1
Plasmid#178758PurposeExpression of a gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E systemDepositorInsertgRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMs_gRNA1
Plasmid#178764PurposeExpression of a gRNA that targets next to PAM library, used for Marinomonas sp. type I-E systemDepositorInsertgRNA that targets next to PAM library, used for Marinomonas sp. type I-E system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-C_array_GG
Plasmid#178767PurposeGolden Gate vector to generate X. albilineans type I-C single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-C single arrays
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc3_gRNA1
Plasmid#178746PurposeExpression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #3 systemDepositorInsertgRNA that targets next to PAM library, used for A. chroococcum type I-E #3 system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLm_gRNA1
Plasmid#178752PurposeExpression of a gRNA that targets next to PAM library, used for L. mobilis type I-E systemDepositorInserta gRNA that targets next to PAM library, used for L. mobilis type I-E system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc2_gRNA1
Plasmid#178740PurposeExpression of a gRNA that targets next to PAM library, used for A. chroococcum type I-E #2 systemDepositorInsertgRNA that targets next to PAM library, used for A. chroococcum type I-E #2 system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66
Plasmid#168787PurposeDonor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.DepositorTypeEmpty backboneUseCre/Lox and Synthetic Biology; Expression in fila…TagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher
Plasmid#157674PurposeExpression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertSpyCatcher with ddFLN4, ELP, Histag and ybbR tag
UseTagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialMutationPromoterT7Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-His-SpyTag-XMod(XL2V)-Doc
Plasmid#157676PurposeExpression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.DepositorInsertXMod-Doc mutant XL2V with Histag and SpyTag
UseTagsHistag and SpyTagExpressionBacterialMutationPromoterT5Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 S389,398 A
Plasmid#129485PurposeExpression in S. cerevisiaeDepositorInsertGlo3 S389,398 A (GLO3 Budding Yeast)
UseTagsExpressionYeastMutationMutation of serines 389 and 398 to alaninePromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Glo3 S389,398 D
Plasmid#129486PurposeExpression in S. cerevisiaeDepositorInsertGlo3 S389,398 D (GLO3 Budding Yeast)
UseTagsExpressionYeastMutationMutation of serines 389 and 398 to AspPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-GAP-BoCCS
Plasmid#123287PurposeExpression of truncated Glo3 (1-375) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation1-375PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-BoCCS-GRM
Plasmid#123288PurposeExpression of truncated Glo3 (137-459) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation137-459PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-Par-BoCCS-GRM
Plasmid#123289PurposeExpression of truncated Glo3 (296-459) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation296-459PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-BoCCS
Plasmid#123290PurposeExpression of truncated Glo3 (137-375) in E.ColiDepositorInsertGlo3 (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutation137-375PromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -