Skip to main content
Addgene
Showing: 2751 - 2800 of 156554 results

  10. mCherry-hPMCA2wb

    Plasmid
    #47752
    Purpose
    mammalian expression of mCherry tagged hPMCA2, wb splice variant
    Depositor
    Emanuel Strehler
    Insert
    PMCA2w/b (ATP2B2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    wb splice variant
    Promoter
    CMV
    Available since
    Nov. 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  11. mCherry-hPMCA2zb

    Plasmid
    #47750
    Purpose
    mammalian expression of mCherry tagged hPMCA2, zb splice variant
    Depositor
    Emanuel Strehler
    Insert
    PMCA2z/b (ATP2B2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    zb splice variant
    Promoter
    CMV
    Available since
    Nov. 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pX330_DRS-1 sgRNA / hSpCas9

    Plasmid
    #172841
    Purpose
    Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172841-plasmid-map-sequence-id-341031

    Enlarge

  21. pET21b(+)-Is-PETase

    Plasmid
    #112202
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12
    Depositor
    Christopher Johnson, Gregg Beckham
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112202-plasmid-map-sequence-id-334619

    Enlarge

  24. pX330_VCL sgRNA / hSpCas9

    Plasmid
    #172837
    Purpose
    Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172837-plasmid-map-sequence-id-340906

    Enlarge

  33. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

    Plasmid
    #225947
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S3E S17E S19E
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225947-plasmid-map-sequence-id-446849

    Enlarge

  34. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

    Plasmid
    #225948
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S3A S17A S19A
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225948-plasmid-map-sequence-id-446850

    Enlarge

  35. pLV-SFFV-CKAP4-WPRE-UbC-Emerald

    Plasmid
    #225943
    Purpose
    Lentiviral vector plasmid expressing human cytoskeleton associated protein 4 (CKAP4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225943-plasmid-map-sequence-id-446819

    Enlarge

  37. pHBJT001

    Plasmid
    #225152
    Purpose
    High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    FLAG epitope tag (35 bp)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225152-plasmid-map-sequence-id-443289

    Enlarge

  43. AAV-sCAG-eGFP-ACAP1 F280E

    Plasmid
    #203801
    Purpose
    AAV vector plasmid expressing human ACAP1 mutation F280E fused to eGFP under a short variant of the CAG (sCAG) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Insert
    ACAP1 (ACAP1 Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    F280E
    Promoter
    Available since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    203801-plasmid-map-sequence-id-407576

    Enlarge

Showing: 2751 - 2800 of 156554 results