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Lenti-sgSmad4#1/Cre

Plasmid

#173617

Purpose

Expresses a Smad4-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Smad4 (Smad4 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDRAV-2

Plasmid

#26747

Depositor

Rolf Zeller

Article

Osterwalder et al Nat Methods. 2010 Oct 17

Type

Empty backbone

Use

Cre/Lox; Flp/frt; phic31/attb-attp

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2011

Availability

Academic Institutions and Nonprofits only

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pBW809_pCAG-PV6-F3-mCherry-BGHpA

Plasmid

#87585

Purpose

PV6 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBSHSP:Cre;CMV:tdTomato-SceI

Plasmid

#30525

Depositor

Gerhart Ryffel

Article

Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

Insert

Cre recombinase tdTomato

Use

Cre/Lox; Heat shock inducible, i-scei megabase ve…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pJW1820

Plasmid

#154321

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC (LoxP)^AID*::3xFLAG

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAW8_6XFLAG

Plasmid

#110235

Purpose

Cre/Lox C-terminal tagging construct encoding 6 copies of the FLAG epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgDnmt3a#1/Cre

Plasmid

#173587

Purpose

Expresses a Dnmt3a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Dnmt3a (Dnmt3a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-spo5DSR

Plasmid

#48983

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#87560

Purpose

Expresses aa397-423 of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa397-423

Use

Cre/Lox and Synthetic Biology

Tags

PYL

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

Plasmid

#87559

Purpose

Expresses aa1-396 of Flp recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa1-396

Use

Cre/Lox and Synthetic Biology

Tags

ABI

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pXP118

Plasmid

#26825

Depositor

Suzanne Sandmeyer, Nancy DaSilva

Article

Fang et al Yeast. 2010 Sep 10. ():.

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV loxP L5-L2

Plasmid

#62094

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site flanked by loxP sites. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Cre/Lox; Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-DIO-mAPX

Plasmid

#79908

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, mAPX

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

mAPX

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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V957 pLPS-3' ECFP-mito - Acceptor

Plasmid

#11699

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

ECFP-mito

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pLPS-hAkt1-gfp

Plasmid

#49388

Purpose

mammalian expression of human Akt1 fused to EGFP

Depositor

Randall McKinnon

Insert

Akt1 (AKT1 Human)

Use

Cre/Lox; Acceptor vector

Tags

EGFP

Expression

Mammalian

Mutation

tga to cca at nt 1725 with respect to GenBank ID …

Promoter

CMV

Available since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only
pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch

Plasmid

#10881

Depositor

Patrick Aebischer

Article

Raoul et al Nat Med. 2005 Apr . 11(4):423-8.

Insert

H1-shSOD1mismatch (SOD1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pH3F

Plasmid

#85773

Purpose

N-ICE plasmid pH3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBW820_pCAG-pExpr-FULL-ADDER

Plasmid

#87552

Purpose

Encodes full adder logic in response to Cre, Flp and VCre recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBS537 tet-hCMV-GFPcre

Plasmid

#11960

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2006

Availability

Academic Institutions and Nonprofits only
pHJ17

Plasmid

#48356

Purpose

Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectable

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR

Use

Cre/Lox; Knockout in t. brucei

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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