Skip to main content
Addgene
Showing: 11141 - 11160 of 24268 results
  1. pEPQD0CM0065

    Plasmid
    #177019
    Purpose
    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT
    Depositor
    Insert
    1-deoxy-D-xylulose 5-phosphate synthase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    transit peptide removed, amplified from cDNA, con…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid
    #173211
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.
    Depositor
    Insert
    ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
    Use
    CRISPR; Prime editing (pe3)
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pRSFDuet1-His-TEV-OTC_d1_Ub

    Plasmid
    #170729
    Purpose
    Scaffold design for studying ubiquitin interacting proteins by cryo-EM.
    Depositor
    Insert
    E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    The C-terminus of OTC was truncated by removing o…
    Promoter
    T7
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. Ash1-5p

    Plasmid
    #169712
    Purpose
    Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed
    Depositor
    Insert
    Ash1 (ASH1 Budding Yeast)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation including encoding amino acids 420-480…
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 11141 - 11160 of 24268 results