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MAC_C_IGF1R

Plasmid

#187770

Purpose

MAC-tagged gene expression of human IGF1R

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

IGF1R (IGF1R Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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p6895 PHAGE-P CMVT N-HA-hE6AP I wt

Plasmid

#37601

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP I wt (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV tet regulatable

Available since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
pDEST40-2XFL-Src-Y419F

Plasmid

#140317

Purpose

Expresses Flag-tagged human Src-Y419F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

Y419F

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAWH

Plasmid

#133894

Purpose

Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GDI2

Plasmid

#67093

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GDI2 (GDI2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pFPL-Gh

Plasmid

#61648

Purpose

Gateway vector for generating green fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced green fluorescent protein

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pUB-cSypHer

Plasmid

#84733

Purpose

Expresses SypHer in plant cells-biosensor for pH

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

SypHer

Use

Tags

Expression

Plant

Mutation

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV NS5

Plasmid

#145358

Purpose

Cell-free expression vector for Zika virus NS5 protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS5

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-Brn2

Plasmid

#32925

Purpose

Retroviral expression of mouse Brn2

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Brn2 (Pou3f2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 IL1B

Plasmid

#67066

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL1B (IL1B Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
p6898 PHAGE-P CMVT N-HA-hE6AP I C820A

Plasmid

#37602

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP I C820A (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C820A

Promoter

CMV tet regulatable

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
PB-Sox17

Plasmid

#60669

Purpose

PiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Sox17 (Sox17 Mouse)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-QUAS5x

Plasmid

#155113

Purpose

5' entry vector containing QUAS5x promoter element.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUAS5x

Use

Gateway 5' entry vector

Tags

Expression

Mutation

Promoter

QUAS5x-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 CIRBP

Plasmid

#67055

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CIRBP (CIRBP Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MAC_C_TOM20

Plasmid

#111678

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TOM20 (TOMM20 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;tmem88aCE1-P1Egfp

Plasmid

#90148

Purpose

contains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

tmem88aE1P1Egfp (tmem88a Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGP8GF-AtTIR1

Plasmid

#188427

Purpose

Arabidopsis Nuclear Auxin Signaling Receptor TIR1

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor TIR1 (TIR1 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-cmlc2-Trpa1b-2A-EGFP

Plasmid

#106405

Purpose

For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Inserts

cmlc2 promoter
Trpa1b (trpa1b Zebrafish)

Use

Gateway

Tags

2A-EGFP

Expression

Mutation

Promoter

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-ngn1-Trpa1b-2A-mCherry

Plasmid

#106425

Purpose

For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Inserts

cmlc2 promoter
Trpa1b (trpa1b Zebrafish)

Use

Gateway

Tags

2A-mCherry

Expression

Mutation

Promoter

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only