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pCXWB-EBNA1

Plasmid

#37624

Purpose

Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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Synapsin sHRPa-NRX in FSW lentiviral vector

Plasmid

#73147

Purpose

Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-NRX

Use

Lentiviral

Tags

2x HA tag and Flag tag

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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Myc-βarrestin2-LOV-Turbo_pCDNA3

Plasmid

#199671

Purpose

expresses Myc-βarrestin2-LOV-Turbo in mammalian cells

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Tags

Myc and βarrestin2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_pSyn_Mito-LOV-Turbo-V5

Plasmid

#199669

Purpose

expresses Mito-LOV-Turbo-V5 in neurons, AAV vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

AAV

Tags

COX4I1 mito targeting sequence and V5

Expression

Mutation

Promoter

Synapsin

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hCaspase-8

Plasmid

#208786

Purpose

Inducibly expresses human Caspase 8 fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase 8 (CASP8 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

CARD domain is removed

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Tags

Expression

Mutation

I319V, N451D, D532N

Promoter

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

Plasmid

#113888

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Use

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEIGW Grx1-roGFP2

Plasmid

#64990

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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Mito-LOV-Turbo-V5

Plasmid

#199666

Purpose

expresses LOV-Turbo in the mammalian mitochondria, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

COX4I1 mito targeting sequence and V5

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hDDX50-HA

Plasmid

#165465

Purpose

Human DDX50 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DExD-box helicase 50 (DDX50 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCW57.1 Lysosomal-METRIQ

Plasmid

#135401

Purpose

Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

DNAse II alpha (DNASE2 Human)

Use

Lentiviral

Tags

T2A, mCherry, and sfGFP

Expression

Mammalian

Mutation

Single-nucleotide polymorphism C432T

Promoter

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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plenti_DCLK1

Plasmid

#163625

Purpose

Expresses DCLK1

Depositor

Kenneth Westover

Article

Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

Insert

DCLK1 (DCLK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

sv40

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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QUIN

Plasmid

#184267

Purpose

A binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.

Depositor

Feng Qu

Article

Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do

Insert

CPSMV RNA1 cDNA with 4 introns

Use

Tags

Expression

Plant

Mutation

S1098P, K1651R

Promoter

CaMV 35S with duplicated enhancer

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLPCX cyto Grx1-roGFP2

Plasmid

#64975

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pGM32DEST

Plasmid

#74747

Purpose

Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

Depositor

Keith Yamamoto, Jordan Ward

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

Gateway cassette
QF-GR (NR3C1 Human, Neurospora crassa, Synthetic)

Use

Tags

Fusion of the Neurospora crassa QF activator to t…

Expression

Worm

Mutation

Promoter

No promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-COX4-dAPEX2

Plasmid

#117176

Purpose

Plasmid name in publication: pAAV-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

COX4-dAPEX2

Use

AAV

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-control-APEX

Plasmid

#73187

Purpose

Expresses "control"-APEX (cytosolic) in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

control-APEX (Nphp3 Glycine max, Mouse)

Use

Tags

internal GFP tag

Expression

Mammalian

Mutation

changed glycine 2 to alanine

Promoter

EF1alpha

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCasso

Plasmid

#207530

Purpose

Conditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sg

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

cytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA

Use

CRISPR; Pseudomonas vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV_CAG_V5-LOV-Turbo-NES

Plasmid

#199670

Purpose

expresses V5-LOV-Turbo-NES in mammalian cells, AAV vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

AAV

Tags

NES and V5

Expression

Mutation

Promoter

CAG

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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