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pHH0103_ITK-1/1

Plasmid

#91253

Purpose

Protein expression and purification of human SH3 domain construct ITK-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITK-1/1 (ITK Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep63

Plasmid

#136811

Purpose

Mammalian expression of the centrosomal protein Cep63 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep63 (CEP63 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Klf4

Plasmid

#172870

Purpose

Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Klf4 (Klf4 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-AGO2 ts2

Plasmid

#115854

Purpose

AGO2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

AGO2 shRNA (AGO2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RIαΔ(D/D+linker)-EGFP

Plasmid

#181843

Purpose

GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of residues 12-112 in PKA RIα.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP Depdc5 pLJM1

Plasmid

#46380

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Differs from Depdc5 isoform 1 by 10 amino acids …

Promoter

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
pZac2.1 gfaABC1D-BioID2-BioID2-Kir4.1-HA

Plasmid

#176746

Purpose

Expresses Kir4.1 fused with BioID2 at the N-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

HA-BioID2-BioID2-Kir4.1 (Kcnj10 Aquifex aeolicus, Rat)

Use

AAV

Tags

HA

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep128

Plasmid

#136823

Purpose

Mammalian expression of the centrosomal protein Cep128 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep128 (CEP128 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAC4 Frk-SH2

Plasmid

#46428

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Frk (FRK Human)

Use

Tags

AviTag, GST, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 108-213

Promoter

Trc

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF9-1/1

Plasmid

#91417

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF9-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF9-1/1 (ARHGEF9 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV NucleoBAS N354K-I372V

Plasmid

#63574

Purpose

Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

Depositor

Stan van de Graaf

Article

van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

Insert

NucleoBAS N354K-I372V

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

N345K and I372V

Promoter

CMV

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only
pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

Plasmid

#192788

Purpose

aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

antiGFP nanobody enhancer

Use

Tags

H6 and ybbR

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

Plasmid

#138524

Purpose

Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

Depositor

Richard Jenner

Article

Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

Insert

PRC2-binding G-tract repeat RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-IRES-puro-3xFLAG-NBR1

Plasmid

#204546

Purpose

Expresses 3xFLAG-tagged NBR1 in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

NBR1 (NBR1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Nsp7

Plasmid

#165112

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp7 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

Plasmid

#157691

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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FXYD2-luc

Plasmid

#31056

Depositor

Gerhart Ryffel

Article

Adalat et al J Am Soc Nephrol. 2009 May . 20(5):1123-

Insert

FXYD2 (FXYD2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

FXYD2 promoter from -2382 to -1

Promoter

Available since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pCDH-EMTB-TurboID-V5-puro

Plasmid

#190741

Purpose

A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

EMTB-TurboID-V5 (RPS14 Human, EMTB is human ensconsin; TurboID is engineered BirA from E.coli)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Microtubule binding-domain of ensconsin (amino ac…

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-DCX-TurboID-V5-puro

Plasmid

#190742

Purpose

A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

DCX-TurboID-V5 (DCX DCX is human doublecortin; TurboID is engineered BirA from E.coli, Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Stat1-SH2

Plasmid

#46511

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat1 (STAT1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 488-683

Promoter

Tac

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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