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pTW082

Plasmid

#115930

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/5 SGP NA (No Aptamer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW083

Plasmid

#115931

Purpose

SGP insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 -98/14 SGP NA (No Aptamer)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS Exon

Plasmid

#110119

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

dati (dati Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mus-Bnip3_deltaExon3

Plasmid

#100793

Purpose

Mammalian expression of Bnip3_deltaExon3 splice variant

Depositor

Joseph Gordon

Article

Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

Insert

mouse-Bnip3_deltaExon3 (Bnip3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Exon3 deletion

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 E-Cad

Plasmid

#67046

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

E-Cad (CDH1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS_K36E_R38E

Plasmid

#65095

Purpose

Destination vector with RBPMS with K36E/R38E mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

change lysine 36 to glutamic acid and arginine 38…

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_R38Q

Plasmid

#65094

Purpose

Destination vector with RBPMS with R38Q mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed arginine 38 to glutamine

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pGMEH-0X750-luxAB-DAS+4

Plasmid

#49519

Purpose

Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomally

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

P750-luxAB-DAS+4

Use

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Mutation

Promoter

P750

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFAB806

Plasmid

#47849

Purpose

BIOFAB reporter plasmid for measuring lambda tR2 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

lambda tR2 terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLTetO4

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB805

Plasmid

#47850

Purpose

BIOFAB reporter plasmid for measuring tonB termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

tonB terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLTetO5

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB816

Plasmid

#47851

Purpose

BIOFAB reporter plasmid for measuring ilvGEDA termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

ilvGEDA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLTetO6

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB807

Plasmid

#47852

Purpose

BIOFAB reporter plasmid for measuring RNAI termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

RNAI terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLTetO7

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB820

Plasmid

#47855

Purpose

BIOFAB reporter plasmid for measuring tetAC termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

tetAC terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLTetO10

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB1910

Plasmid

#47859

Purpose

BIOFAB reporter plasmid for measuring rpoC termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rpoC terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLTetO14

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB804

Plasmid

#47848

Purpose

BIOFAB reporter plasmid for measuring T3 early termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

T3 early terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLTetO3

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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CFP-GAI(1-151)

Plasmid

#37308

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Use

Tags

CFP

Expression

Mammalian

Mutation

contains aa 1-151

Promoter

CMV

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
pNTPTP alpha (S204A)

Plasmid

#17699

Depositor

Xinmin Zheng

Article

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S204A (PTPRA Human)

Use

Tags

Expression

Mammalian

Mutation

S204A

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
lentiGuide-SYNJ2BP-1

Plasmid

#92159

Purpose

CRISPR guide RNA targeting human SYNJ2BP

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP sgRNA-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Availability

Academic Institutions and Nonprofits only

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lentiGuide-SYNJ2BP-2

Plasmid

#92160

Purpose

CRISPR guide RNA targeting human SYNJ2BP

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP sgRNA-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Availability

Academic Institutions and Nonprofits only

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pNEU-hMbPylRS-4xU6M15

Plasmid

#105830

Purpose

Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

Depositor

Irene Coin

Article

Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464

Inserts

Humanized MbPylRS
tRNAM15

Use

Tags

Expression

Mammalian

Mutation

(Y271A, Y349F)MbPylRS

Promoter

CMV and U6

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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