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Plasmid#67090PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP2D6 (CYP2D6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2--3.5ubb:plin2-tdtomato
Plasmid#172534PurposeZebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis markerDepositorInsert-3.5ubb:plin2-tdtomato
UseTagsExpressionMutationS80R, A81S, E144D, I148F and R415H mutations obse…Promoterzebrafish ubbAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Puro DEST (w16-1)
Plasmid#17296Purpose3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, PuroDepositorTypeEmpty backboneUseLentiviral and RNAi; Destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO DEST (775-1)
Plasmid#17432Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoterDepositorTypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEF.DEST51-mScarlet
Plasmid#207960PurposeVector for overexpression of the mScarlet fluorescent proteinDepositorInsertmScarlet
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKH159_FLAG-APEX2-YIPF4 WT
Plasmid#214978PurposeFlag-APEX2 tagged YIPF4DepositorInsertFLAG-APEX2-YIPF4 (YIPF4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKH161_FLAG-APEX2-YIPF3 WT
Plasmid#214980PurposeFlag-APEX2 tagged YIPF3DepositorInsertFLAG-APEX2-YIPF3 (YIPF3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKH143_EGFP-YIPF3 LIR mut
Plasmid#214975PurposeGFP tagged LIR mutant YIPF3DepositorInsertGFP-YIPF3_mut (YIPF3 Human)
UseLentiviralTagsExpressionMutationLIR mutantPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKH142_EGFP-YIPF3 WT
Plasmid#214974PurposeGFP tagged YIPF3DepositorInsertGFP-YIPF3 (YIPF3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-SAR1-V5-His6
Plasmid#67451PurposeMammalian expression of hSar1 with C-term V5 and His6 tagDepositorInsertSAR1 (SAR1A Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-GFP-C1 (w392-1)
Plasmid#17396PurposeGateway entry vector for N-term eGFP fusion, frame C1DepositorInsertGFP
UseEntry vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG
Plasmid#175501PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGFP-P2A-H2AmCherry
Plasmid#170208PurposeDrives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleusDepositorInsertslc1a3b (slc1a3b Zebrafish)
UseZebrafishTagsmyrGFP-P2A-H2AmCherryExpressionMutationPromoterslc1a3bAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pENTR4-GFP-C3 (w393-1)
Plasmid#17397PurposeGateway entry vector for N-term eGFP fusion, frame C3DepositorInsertGFP-C3
UseEntry vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pYPQ167
Plasmid#69309PurposeGateway entry vector with Cas9pDepositorInsertCas9p (Plant codon-optimized; high GC content at 5 prime region)
UseCRISPRTagsExpressionMutationNLS fusionPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S
Plasmid#60815PurposedCas9 with single cysteine residue (M1C) for site-specific labellingDepositorInsertdCas9 M1C D10A C80S H840A C574S
UseTagsHis10 and MBPExpressionBacterialMutationM1C D10A C80S H840A C574SPromoterAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA Null
Plasmid#198728PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.DepositorInsertAtNIA1 gRNAs
UseTagsExpressionPlantMutationPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Klf4-IP
Plasmid#15920DepositorInsertKruppel-like factor 4 (gut) (Klf4 Mouse)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLIX402 PHF2
Plasmid#91700Purposeinducible lentiviral expression of PHF2DepositorInsertPHF2 (PHF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only